CLIC2 Human
Chloride intracellular channel protein 2, XAP121, CLIC2, CLIC2b, XAP121.
Description
CLIC2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 267 amino acids (1-247 a.a.) and having a molecular mass of 30.5kDa.
CLIC2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Chloride intracellular channel protein 2 (CLIC2) plays a crucial role in regulating the movement of chloride ions within cells, a vital process for all living organisms. This protein is involved in various cellular functions, including maintaining stable membrane potential, transmitting signals, regulating cell volume, and transporting organic solutes. CLIC2 is found in fetal liver and adult skeletal muscle tissues. Its location on the X chromosome makes it a potential candidate gene for diseases associated with the Xq28 region.
The CLIC2 protein, produced in E. coli, is a single polypeptide chain that lacks glycosylation. It comprises 267 amino acids, with amino acids 1 to 247 representing the CLIC2 sequence. The protein has a molecular weight of 30.5 kDa.
For purification purposes, a 20 amino acid His-tag is attached to the N-terminus of CLIC2. The protein is then purified using proprietary chromatographic methods.
For short-term storage (2-4 weeks), the CLIC2 solution should be kept at a refrigerated temperature of 4°C. For extended storage, it is recommended to freeze the solution at -20°C.
To ensure stability during long-term storage, it is advisable to add a carrier protein, such as HSA or BSA, at a concentration of 0.1%.
Repeated freezing and thawing of the solution should be avoided.
Chloride intracellular channel protein 2, XAP121, CLIC2, CLIC2b, XAP121.
MGSSHHHHHH SSGLVPRGSH MSGLRPGTQV DPEIELFVKA GSDGESIGNC PFCQRLFMIL WLKGVKFNVT TVDMTRKPEE LKDLAPGTNP PFLVYNKELK TDFIKIEEFL EQTLAPPRYP HLSPKYKESF DVGCNLFAKF SAYIKNTQKE ANKNFEKSLL KEFKRLDDYL NTPLLDEIDP
DSAEEPPVSR RLFLDGDQLT LADCSLLPKL NIIKVAAKKY RDFDIPAEFS GVWRYLHNAY AREEFTHTCP EDKEIENTYA NVAKQKS.
Product Science Overview
CLIC2 is a protein-coding gene that can insert into membranes to form chloride ion channels. The channel activity of CLIC2 is pH-dependent and appears to be regulated by redox conditions, meaning it may only insert into membranes under oxidizing conditions . This protein also modulates the activity of the ryanodine receptor 2 (RYR2) and inhibits calcium influx .
Recombinant human CLIC2 has been extensively studied in various experimental settings. For instance, it has been expressed in HEK cells and studied using patch-clamp techniques. These studies have shown that human CLIC2 can be activated by treatment with forskolin and IBMX, which are known to increase intracellular cAMP levels .
