CLEC2B Human, Sf9
Sf9, Baculovirus cells.
C-Type Lectin Domain Family 2 Member B, C-Type (Calcium Dependent, Carbohydrate-Recognition Domain) Lectin, Superfamily Member 2 (Activation-Induced), IFN-Alpha-2b-Inducing-Related Protein 1, C-Type Lectin Superfamily Member 2, Activation-Induced C-Type Lectin, CLECSF2, IFNRG1, AICL, C-Type Lectin Domain Family 2 Member B, IFN-Alpha2b-Inducing Related Protein 1, HP10085.
Description
CLEC2B produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 366 amino acids (26-149a.a.) and having a molecular mass of 41.7kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
CLEC2B is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques
Product Specs
C-type Lectin Domain Family 2, Member B (CLEC2B) is a protein that belongs to the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The CTL/CTLD superfamily members are known for their diverse functions including cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. They share a common protein fold. The gene encoding CLEC2B is located on chromosome 12p13 in the natural killer gene complex region, closely linked to other CTL/CTLD superfamily members. CLEC2B is predominantly expressed in lymphoid tissues and most hematopoietic cell types.
CLEC2B, a glycosylated polypeptide chain, is produced in Sf9 Baculovirus cells. This recombinant protein consists of 366 amino acids (26-149a.a.) with a molecular mass of 41.7kDa. However, the molecular size on SDS-PAGE may appear between 40-57kDa. The protein is expressed with a 242 amino acid hIgG-His tag at the C-terminus and purified using proprietary chromatographic techniques.
The CLEC2B protein solution is provided at a concentration of 0.25mg/ml and contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
For short-term storage (up to 2-4 weeks), the CLEC2B protein solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. To maintain protein integrity, avoid repeated freeze-thaw cycles.
The purity of CLEC2B is greater than 90.0% as determined by SDS-PAGE analysis.
C-Type Lectin Domain Family 2 Member B, C-Type (Calcium Dependent, Carbohydrate-Recognition Domain) Lectin, Superfamily Member 2 (Activation-Induced), IFN-Alpha-2b-Inducing-Related Protein 1, C-Type Lectin Superfamily Member 2, Activation-Induced C-Type Lectin, CLECSF2, IFNRG1, AICL, C-Type Lectin Domain Family 2 Member B, IFN-Alpha2b-Inducing Related Protein 1, HP10085.
Sf9, Baculovirus cells.
ADPKLTRDSQ SLCPYDWIGF QNKCYYFSKE EGDWNSSKYN CSTQHADLTI IDNIEEMNFL RRYKCSSDHW IGLKMAKNRT GQWVDGATFT KSFGMRGSEG CAYLSDDGAA TARCYTERKW ICRKRIHLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH.
Product Science Overview
C-type lectins are a diverse family of proteins characterized by their ability to bind carbohydrates in a calcium-dependent manner. They play crucial roles in various biological processes, including cell adhesion, cell-cell signaling, glycoprotein turnover, and immune responses. One such member of this family is C-type Lectin Domain Family 2, Member B (CLEC2B), which is encoded by the CLEC2B gene in humans .
The CLEC2B gene is located on chromosome 12p13.31 and encodes a type 2 transmembrane protein. This protein is part of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily, which shares a common protein fold . The CLEC2B protein is expressed on myeloid cells and acts as a ligand to stimulate the activating receptor NKp80/KLRF1 on natural killer (NK) cells . This interaction stimulates NK-cell cytotoxicity and cytokine production, leading to the cytolysis of malignant CLEC2B-expressing myeloid cells .
CLEC2B has diverse functions, including:
- Cell Adhesion: Facilitates the binding of cells to each other or to the extracellular matrix.
- Cell-Cell Signaling: Involved in communication between cells, particularly in the immune system.
- Glycoprotein Turnover: Plays a role in the degradation and recycling of glycoproteins.
- Immune Response: Participates in the activation and regulation of immune cells, particularly NK cells .
The recombinant production of CLEC2B in Sf9 cells involves the use of the baculovirus expression system. Sf9 cells, derived from the fall armyworm (Spodoptera frugiperda), are commonly used for the production of recombinant proteins due to their high expression levels and ability to perform post-translational modifications similar to those in mammalian cells. The process involves the following steps:
- Cloning: The CLEC2B gene is cloned into a baculovirus transfer vector.
- Transfection: Sf9 cells are transfected with the recombinant baculovirus.
- Expression: The transfected cells are cultured, allowing the baculovirus to infect the cells and
