CKMT1A Human

Creatine Kinase, Mitochondrial 1A Human Recombinant
Cat. No.
BT337
Source
E.coli.
Synonyms
Creatine kinase mitochondrial 1A, creatine kinase mitochondrial 1 (ubiquitous), creatine kinase U-type mitochondrial, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, CKMT1, U-MtCK, mia-CK, EC 2.7.3, EC 2.7.3.2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CKMT1A Human Recombinant produced in E. coli is a single polypeptide chain containing 403 amino acids (40-417) and having a molecular mass of 45.0 kDa.
CKMT1A is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The enzyme CKMT1A facilitates the movement of high-energy phosphate from mitochondria to creatine, a cytosolic carrier. Belonging to the creatine kinase isoenzyme family, CKMT1A exists as two isoenzymes: sarcomeric MtCK and ubiquitous MtCK, each encoded by distinct genes. Mitochondrial creatine kinase forms two oligomeric structures, dimers and octamers, unlike cytosolic creatine kinase isoenzymes, which exist exclusively as dimers. Overexpression of ubiquitous mitochondrial creatine kinase has been observed in various malignant cancers with unfavorable prognoses, indicating a potential link to elevated energy metabolism and resistance to apoptosis in cancer cells.
Description
Recombinant human CKMT1A, produced in E. coli, is a single polypeptide chain consisting of 403 amino acids (residues 40-417) with a molecular weight of 45.0 kDa. It includes a 25 amino acid His-tag fused at the N-terminus and is purified through proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CKMT1A solution has a concentration of 1mg/ml and is formulated in 20mM Tris-HCl buffer at pH 8.0, 0.15M NaCl, and 10% glycerol.
Stability
For optimal storage, refrigerate the vial at 4°C if the entire volume will be used within 2-4 weeks. For extended storage, freeze at -20°C. Consider adding a carrier protein such as 0.1% HSA or BSA for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeds 95% as determined by SDS-PAGE analysis.
Biological Activity
The specific activity of the enzyme is greater than 50 units/mg. Specific activity is defined as the amount of enzyme required to catalyze the transfer of 1.0 µmol of phosphate from phosphocreatine to ADP per minute at a pH of 7.5 and a temperature of 37°C.
Synonyms
Creatine kinase mitochondrial 1A, creatine kinase mitochondrial 1 (ubiquitous), creatine kinase U-type mitochondrial, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, CKMT1, U-MtCK, mia-CK, EC 2.7.3, EC 2.7.3.2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMASERR RLYPPSAEYP DLRKHNNCMA SHLTPAVYAR LCDKTTPTGW TLDQCIQTGV DNPGHPFIKT VGMVAGDEET YEVFADLFDP VIQERHNGYD PRTMKHTTDL DASKIRSGYF DERYVLSSRV RTGRSIRGLS LPPACTRAER REVERVVVDA LSGLKGDLAG RYYRLSEMTE AEQQQLIDDH FLFDKPVSPL LTAAGMARDW PDARGIWHNN EKSFLIWVNE EDHTRVISME KGGNMKRVFE RFCRGLKEVE RLIQERGWEF MWNERLGYIL TCPSNLGTGL RAGVHIKLPL LSKDSRFPKI LENLRLQKRG TGGVDTAATG GVFDISNLDR LGKSEVELVQ LVIDGVNYLI DCERRLERGQ DIRIPTPVIH TKH

Product Science Overview

Gene and Protein Structure

CKMT1A is encoded by the CKMT1A gene, which is located on chromosome 15. The gene produces a protein that exists in two isoenzymes: sarcomeric mitochondrial creatine kinase (sMtCK) and ubiquitous mitochondrial creatine kinase (uMtCK). These isoenzymes are encoded by separate genes but share a high degree of homology .

Function and Mechanism

The primary function of CKMT1A is to facilitate the transfer of high-energy phosphate from mitochondria to the cytosolic carrier, creatine. This process is essential for maintaining cellular energy homeostasis, especially in tissues with high and fluctuating energy demands, such as muscles and the brain .

CKMT1A operates in two different oligomeric forms: dimers and octamers. This structural versatility allows it to efficiently manage energy transfer under varying cellular conditions. The enzyme’s activity is crucial for the rapid regeneration of ATP, which is vital for muscle contraction and other energy-intensive cellular processes .

Biological Significance

CKMT1A is highly expressed in tissues with high energy demands, including skeletal muscle, heart muscle, and the brain. Its role in energy metabolism makes it a critical player in various physiological processes. Additionally, overexpression of CKMT1A has been observed in several malignant cancers with poor prognosis, suggesting a potential link between CKMT1A activity and cancer cell metabolism .

Clinical Relevance

Mutations or dysregulation of the CKMT1A gene can lead to metabolic disorders and have been associated with conditions such as mitochondrial complex I deficiency and reticular dysgenesis. The enzyme’s role in energy metabolism also makes it a potential target for therapeutic interventions in metabolic diseases and cancer .

Research and Applications

Human recombinant CKMT1A is used in various research applications to study its function and regulation. Recombinant proteins are produced through genetic engineering techniques, allowing researchers to investigate the enzyme’s properties and interactions in a controlled environment. This research is essential for developing targeted therapies and understanding the enzyme’s role in health and disease .

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