CKMT1A Antibody

Creatine Kinase, Mitochondrial 1A, Mouse Anti Human
Cat. No.
BT15140
Source
Synonyms
Creatine kinase mitochondrial 1A, creatine kinase mitochondrial 1 (ubiquitous), creatine kinase U-type mitochondrial, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, CKMT1, U-MtCK, mia-CK, EC 2.7.3, EC 2.7.3.2.
Appearance
Sterile filtered colorless solution.
Purity
Usage
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Description

Product Specs

Introduction
CKMT1A, a member of the creatine kinase isoenzyme family, facilitates the transfer of high-energy phosphate from mitochondria to the cytosolic carrier, creatine. It exists as two isoenzymes: sarcomeric MtCK and ubiquitous MtCK, each encoded by separate genes. Unlike the exclusively dimeric cytosolic creatine kinase isoenzymes, mitochondrial creatine kinase forms dimers and octamers. Overexpression of ubiquitous mitochondrial creatine kinase, linked to elevated energy turnover and resistance to apoptosis, is observed in various malignant cancers with unfavorable prognoses.
Physical Appearance
A sterile, colorless solution.
Formulation
The solution contains 1mg/ml of CKMT1A antibody in a buffer of PBS at pH 7.4, supplemented with 10% glycerol and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), store the antibody at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has been validated for ELISA and Western blot analysis to ensure specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. A starting dilution of 1:1000 is recommended.
Synonyms
Creatine kinase mitochondrial 1A, creatine kinase mitochondrial 1 (ubiquitous), creatine kinase U-type mitochondrial, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, CKMT1, U-MtCK, mia-CK, EC 2.7.3, EC 2.7.3.2.
Purification Method
CKMT1A antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT17A2AT.
Immunogen
Anti-human CKMT1A mAb, clone PAT17A2AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CKMT1A protein 40-417 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.

Product Science Overview

Introduction

Creatine Kinase, Mitochondrial 1A (CKMT1A) is a protein-coding gene that plays a crucial role in cellular energy metabolism. It is part of the creatine kinase isoenzyme family, which is responsible for the transfer of high-energy phosphate from mitochondria to the cytosolic carrier, creatine .

Gene and Protein Structure

CKMT1A is encoded by the CKMT1A gene, which is located on chromosome 15. The protein exists in two isoenzymes: sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes . The mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes .

Function

CKMT1A plays a central role in energy transduction in tissues with large, fluctuating energy demands such as the brain. It catalyzes the reversible transfer of phosphate between ATP and various phosphagens (e.g., creatine phosphate) . This process is essential for maintaining cellular energy homeostasis, especially in tissues with high energy demands .

Expression and Localization

CKMT1A is ubiquitously expressed in mammals and is mainly localized to the mitochondrial intermembrane space . It is highly expressed in tissues such as the brain, appendix, tonsil, skin, and colon . The protein’s distribution within the cell includes both the mitochondrion and the cytosol .

Clinical Significance

Impairment in CKMT1A due to oxidative and radical-induced damage has been reported in numerous diseases, including neurodegenerative disorders . Overexpression of CKMT1A is observed in several tumors where high energy consumption is required for proliferation . Diseases associated with CKMT1A include Mitochondrial Complex I Deficiency, Nuclear Type 19, and Reticular Dysgenesis .

Mouse Anti Human CKMT1A Antibody

The mouse anti-human CKMT1A antibody is a monoclonal antibody used in various research applications, including Western blotting, immunohistochemistry, and immunocytochemistry . It is used to detect CKMT1A protein expression in human, mouse, and rat tissues . The antibody is purified by affinity chromatography and is available in different concentrations for research purposes .

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Creatine Kinase, Mitochondrial 1A Human Recombinant
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Source
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