CINP Human

Cyclin-Dependent Kinase 2 Interacting Protein (CINP) is a crucial component in the regulation of the cell cycle and DNA replication. This protein, encoded by the CINP gene, plays a significant role in maintaining genome stability and ensuring proper cell division.

The CINP gene is located on chromosome 14 and encodes a protein that is part of the DNA replication complex . The protein interacts with two key kinases, CDK2 and CDC7, providing a functional and physical link between them during the initiation of DNA replication . The interaction with these kinases is essential for the firing of the origins of replication, a critical step in the DNA replication process.

CINP is involved in several critical cellular processes:

1. DNA Replication: CINP binds to chromatin at the G1 phase of the cell cycle and dissociates with the initiation of replication . This binding and dissociation are crucial for the proper timing and regulation of DNA replication.
2. Checkpoint Signaling: CINP regulates ATR-mediated checkpoint signaling in response to DNA damage . This function is vital for maintaining genome integrity and preventing the propagation of damaged DNA.
3. Ribosome Maturation: Recent studies have shown that CINP is also involved in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles .

Mutations or dysregulation of the CINP gene have been associated with several disorders, including microcephaly and Seckel syndrome . These conditions highlight the importance of CINP in normal cellular function and development.

The recombinant form of CINP is used in various research applications to study its role in cell cycle regulation and DNA replication. Understanding the function of CINP can provide insights into the mechanisms of genome maintenance and the development of potential therapeutic targets for diseases related to cell cycle dysregulation.