CIB1 Human
Calcium and Integrin Binding 1 Human Recombinant
Cat. No.
BT28516
Source
Escherichia Coli.
Synonyms
Calcium and integrin-binding protein 1, Calmyrin, DNA-PKcs-interacting protein, Kinase-interacting protein, SNK-interacting protein 2-28, SIP2-28, CIB1, CIB, KIP, PRKDCIP.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CIB1 Human Recombinant is a single, non-glycosylated polypeptide chain (aa 1-191) containing a total of 211 amino acids and having a molecular mass of 23 kDa.
CIB1 is fused to a 20 a.a His-tag and it was purified by conventional column chromatographic techniques.
CIB1 is fused to a 20 a.a His-tag and it was purified by conventional column chromatographic techniques.
Product Specs
Introduction
CIB1, or Calcium and integrin binding 1, is a regulatory protein with a 50% similarity to calmodulin and calcineurin B. This protein belongs to the calcium-binding protein family. CIB1 interacts with DNA-dependent protein kinase and may play a role in the kinase-phosphatase regulation of DNA end joining. CIB1 is also widely expressed and binds to a number of effectors in different tissues, such as integrin βIIb, PAK1, and polo-like kinases.
Description
Recombinant Human CIB1 is a single, non-glycosylated polypeptide chain containing 211 amino acids (aa 1-191) with a molecular weight of 23 kDa. CIB1 is fused to a 20 a.a His-tag and was purified using standard column chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile filtered solution.
Formulation
The CIB1 protein solution (1mg/ml) is supplied in 20mM Tris-HCl, pH 8.0, and 5mM DTT.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, store frozen at -20°C. Adding 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
Calcium and integrin-binding protein 1, Calmyrin, DNA-PKcs-interacting protein, Kinase-interacting protein, SNK-interacting protein 2-28, SIP2-28, CIB1, CIB, KIP, PRKDCIP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGGSGSRLSK ELLAEYQDLT FLTKQEILLA HRRFCELLPQ EQRTVESSLR AQVPFEQILS LPELKANPFK ERICRVFSTS PAKDSLSFED FLDLLSVFSD TATPDIKSHY AFRIFDFDDD GTLNREDLSR LVNCLTGEGE DTRLSASEMK QLIDNILEES DIDRDGTINL SEFQHVISRS PDFASSFKIV L.
Product Science Overview
Gene and Protein Structure
- Gene Location: The CIB1 gene is located on chromosome 15 (15q26.1) in humans .
- Protein Structure: CIB1 is a small protein with a molecular weight of approximately 22 kDa. It contains a conserved calcium-binding EF-hand domain, an integrin-binding domain near the N-terminus, a coiled-coil domain, and a C-terminal domain .
Functions and Interactions
CIB1 plays a crucial role in various cellular processes, including:
- Cell Adhesion and Migration: CIB1 interacts with integrins, which are transmembrane receptors involved in cell signaling and adhesion to the extracellular matrix .
- Cell Survival and Proliferation: It regulates signaling pathways important for cell survival and proliferation .
- Thrombosis and Angiogenesis: CIB1 is involved in thrombopoietin-mediated signaling pathways and negatively regulates alpha-IIb/beta3 activation in thrombin-stimulated megakaryocytes, preventing platelet aggregation .
Clinical Significance
- Cancer: Upregulation of CIB1 expression has been observed in several types of cancer, including breast, lung, prostate, ovarian, and pancreatic cancer. It is implicated in cancer development and progression by regulating cell adhesion, migration, and invasion .
- Alzheimer’s Disease: CIB1 has been associated with Alzheimer’s disease, although the exact mechanisms are still under investigation .
Research and Therapeutic Potential
Given its involvement in critical cellular processes and disease states, CIB1 is a potential target for therapeutic interventions. Research is ongoing to explore its role in various diseases and develop strategies to modulate its activity for therapeutic benefits .
For more detailed information, you can refer to the GeneCards and OMIM entries on CIB1.
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