CIB1 Antibody

Calcium and Integrin Binding 1 (CIB1) is a protein encoded by the CIB1 gene. It is a member of the EF-hand domain-containing calcium-binding superfamily. This protein plays a crucial role in various cellular processes, including cell survival, proliferation, differentiation, and migration. The mouse anti-human CIB1 antibody is a monoclonal antibody derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CIB1 amino acids 1-191 purified from E. coli .

CIB1 is a small protein with a molecular weight of approximately 22 kDa. It has a conserved calcium-binding EF-hand domain, which consists of two alpha-helices connected by a loop. Additionally, CIB1 has an integrin-binding domain located near the N-terminus of the protein, a coiled-coil domain, and a C-terminal domain .

CIB1 interacts with various proteins, including the platelet integrin alpha-IIb-beta-3, DNA-dependent protein kinase, presenilin-2, focal adhesion kinase, p21 activated kinase, and protein kinase D . These interactions suggest that CIB1 is involved in regulating numerous cellular processes, such as cell adhesion, migration, and differentiation .

CIB1 is involved in several critical cellular processes:

• Cell Adhesion and Migration: CIB1 interacts with integrins, which are transmembrane receptors that play a key role in cell signaling and adhesion to the extracellular matrix .
• Cell Survival and Proliferation: CIB1 regulates signaling pathways important for cell survival and proliferation .
• Thrombosis and Angiogenesis: CIB1 plays a role in thrombosis and angiogenesis by negatively regulating alpha-IIb/beta3 activation in thrombin-stimulated megakaryocytes, preventing platelet aggregation .
• Cardiac Hypertrophy and Apoptosis: CIB1 is involved in cardiac hypertrophy and apoptosis, contributing to the regulation of these processes .

CIB1 has been associated with several disease states, including cancer and Alzheimer’s disease . Upregulation of CIB1 expression has been observed in various types of cancer, such as breast, lung, prostate, ovarian, and pancreatic cancer . CIB1 is implicated in cancer progression by regulating cell adhesion, migration, and invasion .

Given its involvement in critical cellular processes and disease states, CIB1 is a potential target for therapeutic interventions. Research on CIB1 continues to explore its role in various diseases and its potential as a therapeutic target .