CETN2 Human
Centrin-2 Human Recombinant
Cat. No.
BT6572
Source
Escherichia Coli.
Synonyms
Centrin-2, Caltractin isoform 1, CETN2, CALT, CEN2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CETN2 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids (1-172 a.a.) and having a molecular mass of 21.9kDa. The CETN2 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Caltractin (CETN2) is a calcium-binding protein and a structural component of the centrosome. It plays a role in nucleotide excision repair by associating with the xeroderma pigmentosum group C protein, suggesting a potential link between cell division and nucleotide excision repair.
Description
Recombinant human CETN2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 192 amino acids (including a 20 amino acid His tag at the N-terminus), encompassing amino acids 1-172 of the CETN2 sequence, and has a molecular weight of 21.9kDa. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The CETN2 is supplied in a solution containing 0.5 mg/ml CETN2, 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Synonyms
Centrin-2, Caltractin isoform 1, CETN2, CALT, CEN2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASNFKKANM ASSSQRKRMS PKPELTEEQK QEIREAFDLF DADGTGTIDV KELKVAMRAL GFEPKKEEIK KMISEIDKEG TGKMNFGDFL TVMTQKMSEK DTKEEILKAF KLFDDDETGK ISFKNLKRVA KELGENLTDE ELQEMIDEAD RDGDGEVSEQ EFLRIMKKTS LY.
Product Science Overview
Overview
Structure and Expression
Centrin-2 is a structural component of the centrosome, which is essential for microtubule organizing center structure and function . The recombinant form of Centrin-2 is typically expressed in Escherichia coli and purified using conventional chromatography techniques . The protein is often tagged with a His-tag at the N-terminus to facilitate purification and detection .
Function
Centrin-2 is involved in several critical cellular functions:
- Centrosome Function: It is required for centriole duplication and correct spindle formation during cell division .
- Cytokinesis and Genome Stability: Centrin-2 plays a role in regulating cytokinesis and maintaining genome stability through its cooperation with other proteins such as CALM1 and CCP110 .
- Nucleotide Excision Repair: It is associated with the xeroderma pigmentosum group C protein, suggesting a role in coupling cell division with nucleotide excision repair .
Applications
Recombinant Human Centrin-2 is used in various research applications, including:
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