CETN1 Human

Centrin-1 Human Recombinant
Cat. No.
BT6487
Source
E.coli.
Synonyms
Centrin EF-hand protein 1, calcium binding protein, Caltractin isoform 2, CETN.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

CETN1 Human Recombinant produced E. coli is a single polypeptide chain containing 196 amino acids (1-172) and having a molecular mass of 22.1kDa.
CETN1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CETN1, a Ca2+-binding protein of the EF-hand family, plays a crucial role in centrosome regulation. It localizes to the centrosome during interphase and undergoes dynamic reorganization at the spindle poles during mitosis, reflecting the centrosome's cell cycle-dependent behavior. CETN1 is essential for centrosome positioning, separation, and microtubule organization.
Description
Recombinant human CETN1, expressed in E. coli, is a single polypeptide chain encompassing 196 amino acids (residues 1-172) with a molecular weight of 22.1 kDa. This protein is engineered with a 24-amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The CETN1 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the solution can be stored at 4°C. For long-term storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of CETN1 is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Centrin EF-hand protein 1, calcium binding protein, Caltractin isoform 2, CETN.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMASGFK KPSAASTGQK RKVAPKPELT EDQKQEVREA FDLFDVDGSG TIDAKELKVA MRALGFEPRK EEMKKMISEV DREGTGKISF NDFLAVMTQK MSEKDTKEEI LKAFRLFDDD ETGKISFKNL KRVANELGEN LTDEELQEMI DEADRDGDGE VNEEEFLRIM KKTSLY

Product Science Overview

Structure and Function

Centrin-1 is a small, calcium-binding protein that contains EF-hand motifs, which are helix-loop-helix structural domains capable of binding calcium ions . This protein plays a crucial role in several cellular processes, including:

  • Determination of Centrosome Position and Segregation: Centrin-1 is involved in the positioning and segregation of centrosomes during cell division .
  • Microtubule Severing: It aids in the severing of microtubules, which is essential for the dynamic reorganization of the cytoskeleton .
  • Cilia Formation: Centrin-1 plays a role in the formation of cilia, which are hair-like structures on the surface of cells that are involved in movement and sensory functions .
Localization and Expression

Centrin-1 is localized to the centrosome in interphase cells and redistributes to the spindle poles during mitosis . This dynamic behavior reflects its involvement in the cell cycle. The protein is highly expressed in tissues such as the sperm, musculoskeletal system, and various glands .

Recombinant Centrin-1

Recombinant Human Centrin-1 is a fragment of the human protein, typically expressed in systems such as wheat germ . It is used in various research applications, including SDS-PAGE, ELISA, and Western Blotting . This recombinant protein is valuable for studying the biological functions and interactions of Centrin-1 in a controlled laboratory setting.

Research and Applications

Research on Centrin-1 has provided insights into its role in cellular processes and its potential implications in diseases. For example, abnormalities in centrosome function and microtubule dynamics are associated with cancer and other diseases. Understanding the function of Centrin-1 can contribute to the development of therapeutic strategies targeting these cellular mechanisms.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

CETN2 Human
Centrin-2 Human Recombinant
Cat. No.
BT6572
Source
Escherichia Coli.
CETN3 Human
Centrin-3 Human Recombinant
Cat. No.
BT6688
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.