CENPU Human

Centromere Protein-U Human Recombinant
Cat. No.
BT7371
Source
Escherichia Coli.
Synonyms
Centromere Protein U, MLF1IP, Polo-Box-Interacting Protein 1, MLF1 Interacting Protein, Centromere Protein Of 50 KDa, Interphase Centromere Complex Protein 24, KLIP1, PBIP1, KSHV Latent Nuclear Antigen-Interacting Protein 1, MLF1-Interacting Protein, CENP50, CENPU50, KSHV Latent Nuclear Antigen Interacting Protein 1, CENP-50, CENP-U, ICEN24.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CENPU Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 295 amino acids (147-418a.a) and having a molecular mass of 33.7kDa.
CENPU is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Centromere Protein U, also known as CENPU, is a specialized chromatin domain present throughout the cell cycle. It serves as a platform for the transient assembly of the kinetochore during mitosis. CENPU plays a crucial role in the correct localization of PLK1 to mitotic kinetochores. Acting as a scaffold protein, CENPU is responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Additionally, CENPU is involved in transcriptional repression.
Description
Recombinant human CENPU, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 295 amino acids (147-418a.a), resulting in a molecular mass of 33.7 kDa. The N-terminus of CENPU is fused to a 23 amino acid His-tag. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
CENPU protein solution at a concentration of 1 mg/ml in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 0.4M Urea.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 80.0% as determined by SDS-PAGE analysis.
Synonyms
Centromere Protein U, MLF1IP, Polo-Box-Interacting Protein 1, MLF1 Interacting Protein, Centromere Protein Of 50 KDa, Interphase Centromere Complex Protein 24, KLIP1, PBIP1, KSHV Latent Nuclear Antigen-Interacting Protein 1, MLF1-Interacting Protein, CENP50, CENPU50, KSHV Latent Nuclear Antigen Interacting Protein 1, CENP-50, CENP-U, ICEN24.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSTRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQP SVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHL EHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKSSLRN AAYFLSNLKQ LYQDYSDVQA QEPNVKETYD SSSLPALLFK ARTLLGAESH LRNINHQLEK LLDQG

Product Science Overview

Structure and Function

CENP-U is part of the constitutive centromere-associated network (CCAN), a complex of proteins that are permanently associated with the centromere throughout the cell cycle. The CCAN is responsible for the assembly and maintenance of the kinetochore, a protein structure on the centromere that attaches chromosomes to the spindle fibers during cell division .

CENP-U interacts with other centromere proteins, such as CENP-C and CENP-H, to form a stable complex that is crucial for kinetochore function. This interaction ensures the proper attachment of spindle fibers to the centromere, facilitating the accurate segregation of chromosomes .

Recombinant CENP-U

Recombinant CENP-U is produced using recombinant DNA technology, which involves inserting the gene encoding CENP-U into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of CENP-U for research and therapeutic purposes.

Recombinant CENP-U is used in various research applications, including the study of centromere and kinetochore function, the investigation of chromosome segregation mechanisms, and the development of potential therapeutic interventions for diseases related to chromosome missegregation .

Clinical Significance

Mutations or dysregulation of CENP-U and other centromere proteins can lead to chromosomal instability, which is a hallmark of many cancers. Understanding the role of CENP-U in chromosome segregation can provide insights into the mechanisms underlying chromosomal instability and potentially lead to the development of targeted therapies for cancer treatment .

Additionally, autoantibodies against centromere proteins, including CENP-U, are often found in patients with autoimmune diseases such as systemic sclerosis and primary biliary cholangitis. These autoantibodies can serve as diagnostic markers and provide insights into the pathogenesis of these diseases .

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