CENPQ Human

CENPQ is a subunit of the CENPH-CENPI-associated centromeric complex. This complex is responsible for targeting CENPA to centromeres, which is necessary for proper kinetochore function and mitotic progression . The kinetochore is a protein structure on the chromosome where the spindle fibers attach during cell division to pull sister chromatids apart.

The human recombinant form of CENPQ is typically produced in E. coli and is often fused with a His-tag at the N-terminus to facilitate purification. The recombinant protein is used in various research applications to study its function and interactions within the centromere complex .

CENPQ plays a significant role in chromosome congression and the recruitment of other centromere proteins such as CENPO, CENPP, and CENPU. It is also involved in the recruitment of CENPE and PLK1 to the kinetochores . These interactions are crucial for the accurate segregation of chromosomes during cell division, ensuring that each daughter cell receives the correct number of chromosomes.

Mutations or malfunctions in CENPQ can lead to various diseases, including spermatogenic failure, which affects male fertility . Understanding the function and structure of CENPQ is essential for developing potential therapeutic strategies for conditions related to chromosome segregation errors.

Recombinant human CENPQ is widely used in research to study its role in the centromere complex. It is utilized in various assays, including SDS-PAGE, to analyze its purity and molecular weight . The recombinant protein is also used to investigate the interactions between CENPQ and other centromere proteins, providing insights into the mechanisms of chromosome segregation.