CENPP Human
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
CENPP is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
CENP-P is part of the constitutive centromere-associated network (CCAN), a complex of proteins that assemble at the centromere. The CCAN is essential for the formation and function of the kinetochore, a structure that attaches chromosomes to spindle microtubules during cell division . CENP-P, along with other CCAN proteins, helps to establish and maintain the centromere-kinetochore interface, which is critical for accurate chromosome segregation .
CENP-P was identified as one of the centromere proteins through studies aimed at understanding the composition and function of the centromere. The protein was found to be intrinsically disordered, meaning it lacks a fixed three-dimensional structure under physiological conditions . This intrinsic disorder allows CENP-P to interact with multiple binding partners and perform its role in the centromere-kinetochore complex.
During cell division, the centromere and kinetochore must function correctly to ensure that chromosomes are evenly distributed to daughter cells. CENP-P, as part of the CCAN, interacts with other centromere proteins to form a stable and functional kinetochore. This interaction is crucial for the attachment of spindle microtubules to the chromosomes, facilitating their movement and segregation .
Recombinant CENP-P is produced using genetic engineering techniques, where the gene encoding CENP-P is inserted into a suitable expression system, such as bacteria or yeast. This allows for the production of large quantities of the protein, which can be used for research and therapeutic purposes. Human recombinant CENP-P is particularly valuable in studying the molecular mechanisms of centromere function and in developing potential treatments for diseases related to chromosome segregation errors, such as cancer.
Research on CENP-P and other centromere proteins has provided significant insights into the mechanisms of chromosome segregation and the role of the centromere in maintaining genomic stability. Understanding how CENP-P functions within the CCAN and kinetochore complex can help identify potential targets for therapeutic intervention in diseases caused by chromosome segregation errors .
