CENPH Human

CENP-H is involved in the assembly of kinetochore proteins, mitotic progression, and chromosome segregation . It colocalizes with inner kinetochore plate proteins CENP-A and CENP-C during both interphase and metaphase . The protein is essential for the localization of CENP-C to the centromere, although it does not affect the localization of CENP-A .

CENP-H is thought to bind to itself and to other centromere proteins such as CENP-A, CENP-B, and CENP-C . Multimers of CENP-H localize constitutively to the inner kinetochore plate, playing a vital role in the organization and function of the active centromere-kinetochore complex .

CENP-H contains a coiled-coil structure and a nuclear localization signal . It localizes outside of centromeric heterochromatin, where CENP-B is found, and inside the kinetochore corona, where CENP-E is localized during prometaphase .

Studies have shown that CENP-H may be associated with certain human cancers . Its role in the proper segregation of chromosomes makes it a critical protein for maintaining genomic stability, and any dysfunction in its activity can lead to chromosomal instability, a hallmark of many cancers .

Recombinant human CENP-H is produced using recombinant DNA technology, which involves inserting the human CENPH gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities . This recombinant protein can be used in various research applications, including studies on kinetochore assembly, mitotic progression, and chromosome segregation.

Recombinant CENP-H is valuable for studying the molecular mechanisms underlying kinetochore function and chromosome segregation. It can be used in biochemical assays to investigate protein-protein interactions, post-translational modifications, and the effects of mutations on protein function . Additionally, it serves as a tool for developing therapeutic strategies targeting centromere-kinetochore dysfunctions in cancer .