CENPB Human
Description
CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Centromere Protein B is a DNA-binding protein derived from transposases of the pogo DNA transposon family . It contains two main functional domains:
- Helix-Loop-Helix DNA Binding Motif: Located at the N-terminus, this domain allows CENP-B to recognize and bind to a specific 17-base pair sequence known as the CENP-B box in the centromeric alpha satellite DNA .
- Dimerization Domain: Located at the C-terminus, this domain facilitates the dimerization of the protein, which is essential for its function in centromere formation .
The primary function of CENP-B is to organize arrays of centromere satellite DNA into a higher-order structure, which then directs the formation of centromeres and the assembly of kinetochores on mammalian chromosomes . This organization is critical for the proper segregation of chromosomes during cell division.
Centromere Protein B is also known as a major centromere autoantigen. It is recognized by sera from patients with anti-centromere antibodies, which are often present in autoimmune diseases such as CREST syndrome and Raynaud’s disease . Additionally, CENP-B has been identified as a potential biomarker for small-cell lung cancer .
Recombinant Centromere Protein B is produced using recombinant DNA technology, which involves inserting the CENPB gene into an expression system to produce the protein in vitro. This recombinant protein is used in various research applications, including the study of centromere structure and function, as well as in diagnostic assays for autoimmune diseases .
Research on CENP-B has provided significant insights into the mechanisms of chromosome segregation and the role of centromeres in maintaining genomic stability. The recombinant form of CENP-B is particularly valuable in these studies, as it allows for detailed biochemical and structural analyses.
