CEACAM3 Human, Sf9

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 3 Human Recombinant, Sf9
Cat. No.
BT4837
Source
Sf9, Baculovirus cells.
Synonyms

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 3, Carcinoembryonic Antigen CGM1, CD66d Antigen , CD66D, CGM1, Carcinoembryonic Antigen Gene Family Member 1, Nonspecific Cross-Reacting Antigen, W264, W282, CEA, CEACAM3.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

CEACAM3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 130 amino acids (35-155a.a.) and having a molecular mass of 14.2kDa (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa).
CEACAM3 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 3, also known as CEACAM3, belongs to the immunoglobulin superfamily. This family comprises genes that encode cell adhesion proteins, often overexpressed in tumor tissues compared to normal tissues. CEACAM3 plays a crucial role in pathogen clearance via the innate immune system. Moreover, it is responsible for stimulating RAC1 during pathogen phagocytosis.
Description
Recombinant Human CEACAM3, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 130 amino acids (35-155a.a.). It exhibits a molecular mass of 14.2kDa (although it may appear between 13.5-18kDa on SDS-PAGE). CEACAM3 is expressed with a C-terminal 6-amino acid His tag and purified using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The CEACAM3 protein solution is provided at a concentration of 1mg/ml in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% as assessed by SDS-PAGE.
Synonyms

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 3, Carcinoembryonic Antigen CGM1, CD66d Antigen , CD66D, CGM1, Carcinoembryonic Antigen Gene Family Member 1, Nonspecific Cross-Reacting Antigen, W264, W282, CEA, CEACAM3.

Source
Sf9, Baculovirus cells.
Amino Acid Sequence

ADPKLTIESM PLSVAEGKEV LLLVHNLPQH LFGYSWYKGE RVDGNSLIVG YVIGTQQATP GAAYSGRETI YTNASLLIQN VTQNDIGFYT LQVIKSDLVN EEATGQFHVY QENAPGLPVG AVAGHHHHHH.

Product Science Overview

Discovery and Structure

CEACAM3 was initially identified as a granulocyte-specific receptor that plays a crucial role in the immune system. It is characterized by its extracellular immunoglobulin (Ig) variable (V)-like domain, which enables both homophilic interactions (binding to itself) and heterophilic interactions (binding to other CEACAM molecules) . This domain structure is essential for its function in mediating cell adhesion and immune responses.

Function and Role in Immunity

CEACAM3 is primarily expressed on granulocytes, a type of white blood cell involved in the body’s defense against bacterial infections. It functions as a decoy receptor for CEACAM1, another member of the CEA family, and mediates the uptake and destruction of specific bacterial pathogens via its immunoreceptor tyrosine-based activation motif (ITAM)-like motif . This process is crucial for the immune system’s ability to recognize and eliminate bacterial invaders.

Clinical Significance

CEACAM3, along with other CEACAM family members, has been studied extensively for its role in cancer progression and metastasis. The expression of CEACAM molecules can be modulated by various factors, including bacterial and viral infections, as well as inflammatory cytokines . This modulation can influence the behavior of cancer cells, making CEACAM3 a potential target for therapeutic interventions in cancer treatment .

Recombinant Production in Sf9 Cells

The recombinant form of CEACAM3, produced in Sf9 insect cells, is used for research and therapeutic purposes. Sf9 cells, derived from the fall armyworm (Spodoptera frugiperda), are commonly used in biotechnology for the production of recombinant proteins. These cells are advantageous for producing glycoproteins like CEACAM3 because they can perform post-translational modifications similar to those in mammalian cells.

Applications in Research and Medicine

Recombinant CEACAM3 is utilized in various research applications, including studies on cell adhesion, immune responses, and cancer biology. It serves as a valuable tool for understanding the molecular mechanisms underlying these processes and for developing potential therapeutic strategies.

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