CDK2AP2 Human

Cyclin-Dependent Kinase 2 Associated Protein 2 Human Recombinant
Cat. No.
BT1027
Source
Escherichia Coli.
Synonyms
Cyclin-dependent kinase 2-associated protein 2, CDK2-associated protein 2, DOC-1-related protein, DOC-1R, CDK2AP2, DOC1R, p14.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CDK2AP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 149 amino acids (1-126 a.a) and having a molecular mass of 15.5kDa.
CDK2AP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cyclin-Dependent Kinase 2 Associated Protein 2 (CDK2AP2), a member of the CDK2AP family, is believed to negatively regulate CDK2 activity. It accomplishes this by sequestering monomeric CDK2 and marking it for proteolysis. CDK2AP2 plays a role in regulating stem cell self-renewal in early embryogenesis. It also regulates self-renewal in mouse embryonic stem cells (mESCs) under specific conditions and influences cell survival during mESC differentiation into specialized cell types.
Description
Recombinant human CDK2AP2, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 149 amino acids (specifically, amino acids 1 through 126) and has a molecular weight of 15.5 kDa. The protein consists of a 23 amino acid His-tag fused to the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CDK2AP2 protein solution has a concentration of 1 mg/ml and contains the following components: 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 50% glycerol, and 2 mM DTT.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
SDS-PAGE analysis indicates a purity greater than 95.0%.
Synonyms
Cyclin-dependent kinase 2-associated protein 2, CDK2-associated protein 2, DOC-1-related protein, DOC-1R, CDK2AP2, DOC1R, p14.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSYKPIA PAPSSTPGSS TPGPGTPVPT GSVPSPSGSV PGAGAPFRPL FNDFGPPSMG YVQAMKPPGA QGSQSTYTDL LSVIEEMGKE IRPTYAGSKS AMERLKRGII HARALVRECL AETERNART.

Product Science Overview

Gene and Protein Structure

The CDK2AP2 gene is located on chromosome 11 and encodes a protein that interacts with cyclin-dependent kinase 2 (CDK2). The protein is a component of the histone deacetylase NuRD complex, which is involved in chromatin remodeling . The NuRD complex is essential for regulating gene expression by modifying the structure of chromatin, making it either more or less accessible for transcription.

Function and Mechanism

CDK2AP2 inhibits the cell cycle G1/S phase transition by repressing CDK2 expression and activation . It achieves this by inhibiting the interaction of CDK2 with cyclins E and A, which are necessary for the progression of the cell cycle . This repression is crucial for maintaining proper cell cycle control and preventing uncontrolled cell proliferation, which can lead to cancer.

Additionally, CDK2AP2 plays a role in regulating the self-renewal of embryonic stem cells and maintaining cell survival during their terminal differentiation . It also regulates microtubule organization in metaphase II oocytes, which is important for proper cell division .

Clinical Significance

Mutations or dysregulation of the CDK2AP2 gene have been associated with various diseases, including Friedreich Ataxia . This association highlights the importance of CDK2AP2 in maintaining normal cellular functions and its potential role in disease development.

Research and Applications

Human recombinant CDK2AP2 is used in research to study its role in cell cycle regulation and chromatin remodeling. Understanding the function and regulation of CDK2AP2 can provide insights into the mechanisms of cell cycle control and the development of cancer. Additionally, it can aid in the development of therapeutic strategies targeting CDK2AP2 for the treatment of diseases associated with its dysregulation.

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Source
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