CDK2AP1 Human

Cyclin-Dependent Kinase 2 Associated Protein 1 Human Recombinant
Cat. No.
BT937
Source
Escherichia Coli.
Synonyms
Cyclin-dependent kinase 2-associated protein 1, CDK2-associated protein 1, Putative oral cancer suppressor, Deleted in oral cancer 1, DOC-1, CDK2AP1, CDKAP1, DOC1, ST19, DORC1, p12DOC-1.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CDK2AP1 Human Recombinant fused to 37 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 152 amino acids (1-115 a.a.) and having a molecular mass of 16.6 kDa. The CDK2AP1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
CDK2AP1, a CDK2-associated protein, negatively regulates CDK2 activity through CDK2 monomer sequestration and targeted proteolysis. Its interaction with DNA polymerase alpha facilitates phosphorylation of the p180 subunit, suggesting a regulatory role in S phase DNA replication.
Description
Recombinant human CDK2AP1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This 16.6 kDa protein consists of 152 amino acids, including a 37 amino acid His Tag at the N-terminus (1-115 a.a. of CDK2AP1). Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The CDK2AP1 solution is formulated in 20mM Tris-HCl (pH 8.0), 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the solution can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
Cyclin-dependent kinase 2-associated protein 1, CDK2-associated protein 1, Putative oral cancer suppressor, Deleted in oral cancer 1, DOC-1, CDK2AP1, CDKAP1, DOC1, ST19, DORC1, p12DOC-1.
Source
Escherichia Coli.
Amino Acid Sequence
RGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSY KPNLAAHMPA AALNAAGSVH SPSTSMATSS QYRQLLSDYG PPSLGYTQGT GNSQVPQSKY AELLAIIEEL GKEIRPTYAG SKSAMERLKR GIIHARGLVR ECLAETERNA RS.

Product Science Overview

Introduction

Cyclin-Dependent Kinase 2 Associated Protein 1 (CDK2AP1), also known as p12DOC-1 or doc-1 (deleted in oral cancer-1), is a highly conserved and ubiquitously expressed gene located on chromosome 12q24. This gene encodes a 115-amino acid protein that plays a crucial role in regulating the cell cycle and epigenetic mechanisms.

Function and Mechanism

CDK2AP1 is primarily known for its role as a negative regulator of Cyclin-Dependent Kinase 2 (CDK2). It achieves this by sequestering monomeric CDK2 and targeting it for proteolysis . This regulation is essential for maintaining proper cell cycle progression, particularly during the S-phase, where DNA replication occurs .

Additionally, CDK2AP1 interacts with DNA polymerase alpha/primase and mediates the phosphorylation of the large p180 subunit, suggesting a regulatory role in DNA replication . This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NuRD) complex, which epigenetically regulates embryonic stem cell differentiation .

Clinical Significance

CDK2AP1 has been implicated in various malignancies, including oral cancer and prostate cancer . Its downregulation has been observed in several types of cancer, indicating its potential role as a tumor suppressor . In prostate cancer, high CDK2AP1 expression has been associated with worse overall survival and cause-specific survival .

Research and Applications

Human recombinant CDK2AP1 is utilized in research to study its role in cell cycle regulation and its potential as a therapeutic target in cancer treatment. By understanding the mechanisms through which CDK2AP1 regulates CDK2 and other cellular processes, researchers aim to develop novel strategies for cancer therapy.

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