CDC37 Antibody
Description
Product Specs
Product Science Overview
Cell Division Cycle 37 (CDC37) is a protein that plays a crucial role in cell cycle regulation and protein folding. It is a co-chaperone that works in conjunction with Heat Shock Protein 90 (Hsp90) to stabilize and fold various client proteins, particularly kinases . CDC37 is essential for the proper functioning of these proteins, which are involved in critical cellular processes such as signal transduction, cell growth, and division .
CDC37 is composed of several domains that facilitate its interaction with Hsp90 and client proteins. The N-terminal domain of CDC37 is responsible for binding to client proteins, while the middle domain interacts with Hsp90. The C-terminal domain is involved in the dimerization of CDC37, which is necessary for its function .
CDC37 specifically targets protein kinases, which are enzymes that modify other proteins by adding phosphate groups. This modification is crucial for regulating protein activity, localization, and interactions. By stabilizing these kinases, CDC37 ensures that they are properly folded and functional, thereby maintaining cellular homeostasis .
- Western Blotting: Mouse anti-human CDC37 antibodies are commonly used in Western blotting to detect CDC37 protein levels in cell lysates. This technique helps researchers study the expression and regulation of CDC37 in different cell types and under various conditions .
- Immunofluorescence: These antibodies can also be used in immunofluorescence assays to visualize the localization of CDC37 within cells. By tagging the antibodies with fluorescent dyes, researchers can observe the distribution of CDC37 in different cellular compartments .
- Immunoprecipitation: Mouse anti-human CDC37 antibodies are useful for immunoprecipitation experiments, where they are used to isolate CDC37 and its interacting proteins from cell lysates. This allows researchers to study the protein-protein interactions involving CDC37 and identify its client proteins .
