CD9 Human, HEK
HEK293 Cells.
CD9 antigen, BA2, BTCC-1, DRAP-27, MRP-1, MIC3, TSPAN-29, TSPAN29, 5H9 antigen,Cell growth-inhibiting gene 2 protein,Leukocyte antigen MIC3,p24,CD9 antigen isoform1.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD9 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 93 amino acids (112-195a.a) and having a molecular mass of 10.7kDa.
CD9 is fused to 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD9, a cell surface glycoprotein found on exosomes, interacts with integrins and transmembrane superfamily proteins. It plays a role in platelet activation, aggregation, and paranodal junction formation. Additionally, CD9 participates in cell adhesion, migration, and muscle cell fusion. It is essential for egg-sperm fusion during mammalian fertilization.
Recombinant Human CD9, produced in HEK293 cells, is a single, glycosylated polypeptide chain consisting of 93 amino acids (112-195a.a) with a molecular weight of 10.7kDa. It is fused to a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic methods.
Sterile, colorless solution.
The CD9 solution is provided at a concentration of 1mg/ml in phosphate-buffered saline (pH 7.4) containing 10% glycerol.
For short-term storage (2-4 weeks), store at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity greater than 90.0% as determined by SDS-PAGE analysis.
CD9 antigen, BA2, BTCC-1, DRAP-27, MRP-1, MIC3, TSPAN-29, TSPAN29, 5H9 antigen,Cell growth-inhibiting gene 2 protein,Leukocyte antigen MIC3,p24,CD9 antigen isoform1.
HEK293 Cells.
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Product Science Overview
CD9, also known as Tetraspanin-29, is a cell surface glycoprotein that belongs to the transmembrane 4 superfamily, commonly referred to as the tetraspanin family . This protein is characterized by its four transmembrane domains and two extracellular loops, which contain conserved disulfide bonds . CD9 plays a crucial role in various cellular processes, including cell adhesion, migration, and signal transduction.
CD9 is a member of the tetraspanin family, which is known for its role in organizing microdomains on the cell membrane . These microdomains facilitate interactions between various cell surface proteins, including integrins and other tetraspanins . CD9 is involved in several cellular functions:
- Cell Adhesion: CD9 interacts with integrins to promote cell adhesion, which is essential for tissue formation and maintenance .
- Signal Transduction: CD9 participates in signal transduction pathways that regulate cell proliferation, differentiation, and migration .
- Immune Response: CD9 is expressed on the surface of various immune cells and plays a role in immune cell signaling and function .
Recombinant CD9 (Human Recombinant, HEK) is produced using human embryonic kidney (HEK) 293 cells . The process involves the following steps:
- Gene Cloning: The gene encoding human CD9 is cloned into an expression vector.
- Transfection: The expression vector is introduced into HEK293 cells, which are known for their high transfection efficiency and ability to produce recombinant proteins with human-like post-translational modifications.
- Protein Expression: The transfected HEK293 cells express the recombinant CD9 protein, which is then harvested from the cell culture.
- Purification: The recombinant CD9 protein is purified using techniques such as affinity chromatography to achieve high purity levels (>95% as determined by SDS-PAGE) .
Recombinant CD9 has several applications in research and medicine:
- Cancer Research: CD9 is a marker for various cancers, and its expression levels can be used to study cancer progression and metastasis .
- Immunology: CD9 is involved in immune cell signaling, making it a valuable tool for studying immune responses and developing immunotherapies .
- Cell Biology: CD9’s role in cell adhesion and migration makes it an important protein for studying cell behavior and tissue formation .
