CD9 Human, Sf9

CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. This family of proteins is characterized by four hydrophobic domains and is involved in various cellular processes, including cell adhesion, motility, and signal transduction .

CD9 is a cell surface glycoprotein that plays a crucial role in the immune system. It is involved in the formation of complexes with integrins and other tetraspanin family members. These complexes are essential for various cellular functions, including cell adhesion and migration .

Recombinant CD9 (Human) is produced using the Sf9 insect cell expression system. The DNA sequence encoding the second extracellular domain (Ser112-Ile195) of human CD9 is fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus. This recombinant protein is expressed in HEK293 cells and is purified to a high degree of purity (>95%) as determined by SDS-PAGE .

Recombinant CD9 is used in various research applications, including:

• Cell signaling studies: Understanding the role of CD9 in signal transduction pathways.
• Cell adhesion research: Investigating the mechanisms of cell adhesion and migration.
• Immunophenotyping: Identifying different cell types in the immune system based on CD9 expression .

Recombinant CD9 is typically provided as a lyophilized powder and is stable for up to twelve months when stored at -20°C to -80°C. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles, which can affect its stability and activity .