CD8B Human, Sf9
CD8b Molecule, CD8 Antigen, Beta Polypeptide 1 (P37), CD8B1, T Lymphocyte Surface Glycoprotein Beta Chain, T-Cell Surface Glycoprotein CD8 Beta Chain, CD8b Antigen, Leu2, LYT3, P37, LY3, T-cell surface glycoprotein CD8 beta chain.
Greater than 85.0% as determined by SDS-PAGE.
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Description
CD8B produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 158 amino acids (22-170a.a.) and having a molecular mass of 17.8kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
CD8B is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The CD8 antigen is encoded in its beta chain isoforms by the CD8B gene. Located on most cytotoxic T lymphocytes, CD8 is a cell surface glycoprotein that facilitates efficient cell-cell interactions within the immune system. Antigens displayed by an antigen-presenting cell (APC) are identified by the T-cell receptor on the T lymphocyte and the co-receptor CD8 antigen in the context of class I MHC molecules. A heterodimer made up of one alpha and one beta chain or a homodimer made up of two alpha chains can act as the functional co-receptor. Both the alpha and beta chains share significant homology with immunoglobulin variable light chains.
Produced in Sf9 Baculovirus cells, CD8B is a single, glycosylated polypeptide chain with a molecular weight of 17.8 kDa that contains 158 amino acids (22-170a.a.). (On SDS-PAGE, the molecular size will fall between 18 and 28 kDa). A 6 amino acid His tag is present at the C-terminus of CD8B, which is purified using proprietary chromatographic methods.
The CD8B protein solution (0.5 mg/ml) is supplied in 10% glycerol and Phosphate Buffered Saline (pH 7.4).
SDS-PAGE analysis determined purity to be greater than 85%.
CD8b Molecule, CD8 Antigen, Beta Polypeptide 1 (P37), CD8B1, T Lymphocyte Surface Glycoprotein Beta Chain, T-Cell Surface Glycoprotein CD8 Beta Chain, CD8b Antigen, Leu2, LYT3, P37, LY3, T-cell surface glycoprotein CD8 beta chain.
ADPLQQTPAY IKVQTNKMVM LSCEAKISLS NMRIYWLRQR QAPSSDSHHE FLALWDSAKG TIHGEEVEQE KIAVFRDASR FILNLTSVKP EDSGIYFCMI VGSPELTFGK GTQLSVVDFL PTTAQPTKKS TLKKRVCRLP RPETQKGPLC SPHHHHHH.
Product Science Overview
The CD8 molecule is typically expressed as a heterodimer consisting of an alpha (CD8α) and a beta (CD8β) chain. The CD8αβ heterodimer functions as a coreceptor with the T cell receptor (TCR), enhancing the TCR’s ability to recognize antigens presented by major histocompatibility complex class I (MHC-I) molecules on the surface of antigen-presenting cells (APCs) . This interaction is critical for the activation and function of cytotoxic T cells, which are responsible for killing infected or cancerous cells.
The human CD8B gene is located on chromosome 2 and encodes several alternatively spliced variants with different cytoplasmic tails (M-1, M-2, M-3, and M-4) . These isoforms have distinct expression patterns and functional roles in T cell responses. For example, the M-2 isoform is upregulated upon T cell activation and is involved in the regulation of TCR signaling through ubiquitination .
Recombinant CD8B protein can be produced using various expression systems, including Sf9 insect cells. The Sf9 cell line, derived from the fall armyworm (Spodoptera frugiperda), is commonly used for the production of recombinant proteins due to its high expression levels and ability to perform post-translational modifications . The recombinant CD8B protein produced in Sf9 cells is often tagged with a His-tag to facilitate purification and detection .
Recombinant CD8B protein is used in various research applications, including studies on T cell function, immune responses, and the development of immunotherapies. By understanding the role of CD8B in T cell activation and signaling, researchers can develop new strategies to enhance immune responses against infections and cancers .
