CD80 Mouse

CD80, also known as B7-1, is a protein that plays a crucial role in the activation of T cells. It is a member of the immunoglobulin superfamily and is primarily expressed on the surface of antigen-presenting cells (APCs) such as B cells, macrophages, and dendritic cells. CD80 interacts with its receptors, CD28 and CTLA-4, to regulate immune responses. The recombinant form of CD80 (Mouse) is produced using various expression systems for research and therapeutic purposes.

CD80 consists of an extracellular domain with two immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic domain. The extracellular domain is responsible for binding to its receptors, CD28 and CTLA-4. While CD28 binding leads to T cell activation and proliferation, CTLA-4 binding results in the downregulation of the immune response. This dual functionality makes CD80 a critical molecule in maintaining immune homeostasis .

Recombinant CD80 (Mouse) is typically produced using mammalian cell expression systems, such as HEK293 cells. The gene encoding CD80 is cloned into an expression vector, which is then transfected into the host cells. The cells are cultured, and the protein is harvested from the culture supernatant. The recombinant protein is purified using techniques such as affinity chromatography, ensuring high purity and functionality .

Recombinant CD80 (Mouse) is widely used in immunological research to study T cell activation and co-stimulation. It is also utilized in the development of immunotherapies for diseases such as cancer and autoimmune disorders. By understanding the interactions between CD80 and its receptors, researchers can design targeted therapies to modulate immune responses .