CD80 Antibody
Description
Product Specs
Product Science Overview
B7-1, also known as CD80, is a protein that plays a crucial role in the immune system. It is a member of the immunoglobulin superfamily and is primarily expressed on the surface of activated B cells, dendritic cells, and monocytes. The interaction between B7-1 and its receptors is essential for the activation and regulation of T cells, which are vital for the adaptive immune response.
B7-1 is a 60 kDa glycoprotein that consists of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain is responsible for binding to its receptors, CD28 and CTLA-4. B7-1 is expressed on the surface of antigen-presenting cells (APCs) such as dendritic cells, macrophages, and activated B cells. Its expression is upregulated in response to inflammatory signals and during immune responses.
The primary function of B7-1 is to provide costimulatory signals necessary for T cell activation and survival. When an APC presents an antigen to a T cell, the interaction between the T cell receptor (TCR) and the antigen-MHC complex is not sufficient for full T cell activation. The binding of B7-1 to CD28 on the T cell provides a crucial second signal that promotes T cell proliferation, cytokine production, and survival. Conversely, the interaction between B7-1 and CTLA-4 delivers an inhibitory signal that downregulates T cell responses, maintaining immune homeostasis and preventing autoimmunity.
Rat anti-mouse B7-1 (CD80) antibodies are monoclonal antibodies developed in rats that specifically target the mouse B7-1 protein. These antibodies are widely used in research to study the role of B7-1 in immune responses, as well as in various immunological assays such as flow cytometry, immunohistochemistry, and Western blotting.
- Flow Cytometry: Rat anti-mouse B7-1 antibodies are used to detect and quantify B7-1 expression on the surface of cells. This technique is valuable for studying the activation status of APCs and the regulation of immune responses.
- Immunohistochemistry: These antibodies are used to visualize B7-1 expression in tissue sections. This application helps researchers understand the spatial distribution of B7-1 in different tissues and its role in various physiological and pathological conditions.
- Western Blotting: Rat anti-mouse B7-1 antibodies are used to detect B7-1 protein levels in cell lysates or tissue extracts. This technique is useful for studying the regulation of B7-1 expression and its involvement in immune signaling pathways.
