CD79B Human

CD79B Human Recombinant
Cat. No.
BT30804
Source
E.coli.
Synonyms
AGM6, B29, IGB, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, B-cell-specific glycoprotein B29, Immunoglobulin-associated B29 protein, CD79b, CD antigen, CD79b.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CD79B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids (29-159a.a) and having a molecular mass of 17.7kDa.
CD79B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD79B, a 36 kDa transmembrane glycoprotein, belongs to the immunoglobulin superfamily. It forms heterodimers with CD79A, which then bind to a membrane-bound immunoglobulin on B cells. This complex constitutes the B cell antigen receptor (BCR). Both CD79A and CD79B are crucial for BCR signaling, playing a vital role in the development and activation of B lineage cells.
Description
Recombinant human CD79B, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 155 amino acids (29-159a.a), resulting in a molecular weight of 17.7 kDa. The protein includes a 24 amino acid His-tag at the N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterilized by filtration.
Formulation
The CD79B protein solution is supplied at a concentration of 1 mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 2M urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep at 4°C. For longer periods, store frozen at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90%, as determined by SDS-PAGE analysis.
Synonyms
AGM6, B29, IGB, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, B-cell-specific glycoprotein B29, Immunoglobulin-associated B29 protein, CD79b, CD antigen, CD79b.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMARSEDR YRNPKGSACS RIWQSPRFIA RKRGFTVKMH CYMNSASGNV SWLWKQEMDE NPQQLKLEKG RMEESQNESL ATLTIQGIRF EDNGIYFCQQ KCNNTSEVYQ GCGTELRVMG FSTLAQLKQR NTLKD.

Product Science Overview

Introduction

CD79B, also known as B29, Ig beta, or B cell antigen receptor complex-associated protein beta-chain, is a crucial component of the B cell receptor (BCR) complex. It is a member of the immunoglobulin superfamily and plays a significant role in the development and function of B cells. CD79B forms a heterodimer with CD79A (Ig alpha), and this complex is essential for the signal transduction of the BCR, which is vital for B cell activation and antibody production .

Structure and Function

CD79B is a transmembrane protein with a molecular weight of approximately 36-40 kDa. It consists of an extracellular immunoglobulin-like domain, a single transmembrane region, and a cytoplasmic tail containing immunoreceptor tyrosine-based activation motifs (ITAMs). These ITAMs are critical for initiating intracellular signaling cascades upon antigen binding to the BCR .

The CD79A/CD79B heterodimer associates non-covalently with membrane-bound immunoglobulins (IgM, IgD, IgG, IgA, or IgE) to form the BCR complex. This complex is responsible for recognizing specific antigens and triggering B cell activation, proliferation, and differentiation .

Recombinant CD79B

Recombinant CD79B is produced using various expression systems, such as Chinese Hamster Ovary (CHO) cells or HEK293 cells. The recombinant protein is typically purified to high levels of purity, often exceeding 95%, and is used in various research applications, including structural studies, functional assays, and therapeutic development .

Applications in Research and Medicine

CD79B is widely studied in the context of B cell malignancies, such as B cell lymphomas and leukemias. It is a target for chimeric antigen receptor (CAR) T cell therapies, which are engineered to recognize and kill cancerous B cells expressing CD79B . Additionally, CD79B is used in the development of monoclonal antibodies and other therapeutic agents aimed at modulating B cell function in autoimmune diseases and other conditions .

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