CD72 Human

CD72 consists of a 95 amino acid cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs), a 21 amino acid transmembrane segment, and a 243 amino acid extracellular domain that includes a coiled-coil domain and a C-type lectin domain . The ITIMs in the cytoplasmic domain are essential for its inhibitory functions, which help in the negative regulation of BCR signaling.

Recombinant human CD72 is produced using various expression systems, such as HEK293 cells . The recombinant protein typically includes a polyhistidine tag at the C-terminus to facilitate purification. The recombinant human CD72 consists of 254 amino acids and has a predicted molecular mass of approximately 29.53 kDa, although it migrates as an approximately 34.46 kDa band in SDS-PAGE under reducing conditions .

Recombinant CD72 is used in various research applications, including studies on immune regulation, autoimmune diseases, and cancer. It is particularly valuable in investigating the mechanisms of B cell regulation and the role of CD72 in maintaining immune homeostasis . Additionally, CD72 has been identified as a potential prognostic biomarker in certain cancers, such as kidney renal clear cell carcinoma (KIRC) .

Recombinant CD72 is typically provided as a lyophilized powder and should be stored at -20°C to -80°C under sterile conditions to maintain its stability . It is recommended to avoid repeated freeze-thaw cycles to preserve the protein’s activity.