CD72 Human, Sf9
Sf9, Baculovirus cells.
CD72, CD72b, LYB2, B-cell differentiation antigen CD72, Lyb-2.
Greater than 90% as determined by SDS-PAGE.
Description
CD72 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 485 amino acids (117-359a.a.) and having a molecular mass of 55.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
CD72 is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD72, a surface antigen found on B cells, belongs to a family of type II integral membrane glycoproteins. This family includes molecules like asialoglycoprotein receptors, CD23, and the Kupffer cell receptor. When B cells are exposed to antibodies targeting CD72, various signaling pathways are activated. This activation can lead to increased MHC class II expression and stimulate B cell proliferation. CD72 is present on most B cell types except for plasma cells and is weakly expressed on macrophages in human tissues.
This product consists of a recombinant human CD72 protein produced in Sf9 insect cells using a baculovirus expression system. The protein is a single, glycosylated polypeptide chain containing 485 amino acids (specifically, amino acids 117 to 359). It has a molecular weight of 55.3 kDa. When analyzed using SDS-PAGE, the protein appears as a band in the range of 50-70 kDa. This recombinant CD72 protein includes a 242 amino acid hIgG-His tag located at the C-terminus. The protein is purified to a high degree using proprietary chromatographic techniques.
The CD72 protein is supplied in a solution with a concentration of 0.5 mg/ml. The solution also contains 50mM Tris-HCl buffer (pH 6.8), 0.2M NaCl, 2mM DTT, and 50% glycerol.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein like HSA or BSA to a final concentration of 0.1%. It is important to avoid repeated freezing and thawing of the product.
The purity of the CD72 protein in this product is greater than 90% as determined by SDS-PAGE analysis.
CD72, CD72b, LYB2, B-cell differentiation antigen CD72, Lyb-2.
Sf9, Baculovirus cells.
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Product Science Overview
CD72 is expressed on all cells of the B cell lineage, except for plasma cells, and is weakly expressed on human tissue macrophages . The human recombinant CD72 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 485 amino acids (117-359a.a.) and has a molecular mass of approximately 55.3 kDa . The molecular size on SDS-PAGE appears at approximately 50-70 kDa .
The primary function of CD72 is not entirely understood, but it is known to be involved in B cell activation and proliferation. When B cells are exposed to CD72 antibodies, a variety of signaling pathways are activated, leading to the induction of MHC class II expression and B cell proliferation . This makes CD72 an important molecule in the regulation of immune responses.
The recombinant CD72 protein is produced in Sf9 Baculovirus cells and is purified using proprietary chromatographic techniques . The protein is expressed with a 242 amino acid hIgG-His tag at the C-terminus, which aids in its purification . The final product is a sterile, filtered, colorless solution formulated in 50mM Tris-HCl buffer (pH 6.8), 0.2M NaCl, 2mM DTT, and 50% glycerol .
Recombinant CD72 is used in various laboratory research applications, including ELISA, inhibition assays, and Western blotting . It is important to note that ProSpec’s CD72 products are intended for laboratory research use only and are not suitable for use as drugs, agricultural or pesticidal products, food additives, or household chemicals .
For short-term storage, CD72 should be kept at 4°C if the entire vial will be used within 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C and to add a carrier protein (0.1% HSA or BSA) to prevent degradation . It is crucial to avoid multiple freeze-thaw cycles to maintain the protein’s stability .
