CD5 Human
CD5 Human Recombinant
Cat. No.
BT29969
Source
E.coli.
Synonyms
CD5 Molecule, CD5, LEU1, CD5 Antigen (P56-62), Lymphocyte Antigen T1/Leu-1, T1, T-Cell Surface Glycoprotein CD5, CD5 Antigen.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CD5 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 371 amino acids (25-372) and having a molecular mass of 41.0 kDa.
CD5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CD5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
CD5 is a useful marker for T-cells in immunohistochemistry, although not as sensitive as CD3. It is expressed in about 76% of T-cell neoplasms and is also found in chronic lymphocytic leukemia, hairy cell leukemia, and mantle cell lymphoma cells. The loss of CD5 is frequently seen in cutaneous T-cell lymphoma, and its absence can be a sign of malignancy. In T cell acute lymphoblastic leukemia, lack of CD5 expression, while uncommon, is associated with a poor prognosis. As a receptor, CD5 plays a role in regulating T-cell proliferation.
Description
Recombinant human CD5, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It encompasses amino acids 25-372, resulting in a molecular weight of 41.0 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CD5 solution is provided at a concentration of 1mg/ml in a buffer composed of 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M Urea.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 80% by SDS-PAGE analysis.
Synonyms
CD5 Molecule, CD5, LEU1, CD5 Antigen (P56-62), Lymphocyte Antigen T1/Leu-1, T1, T-Cell Surface Glycoprotein CD5, CD5 Antigen.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSRLSWYDP DFQARLTRSN SKCQGQLEVY LKDGWHMVCS QSWGRSSKQW EDPSQASKVC QRLNCGVPLS LGPFLVTYTP QSSIICYGQL GSFSNCSHSR NDMCHSLGLT CLEPQKTTPP TTRPPPTTTP EPTAPPRLQL VAQSGGQHCA GVVEFYSGSL GGTISYEAQD KTQDLENFLC NNLQCGSFLK HLPETEAGRA QDPGEPREHQ PLPIQWKIQN SSCTSLEHCF RKIKPQKSGR VLALLCSGFQ PKVQSRLVGG SSICEGTVEV RQGAQWAALC DSSSARSSLR WEEVCREQQC GSVNSYRVLD AGDPTSRGLF CPHQKLSQCH ELWERNSYCK KVFVTCQDPN P.
Product Science Overview
Structure and Expression
CD5 is a 67 kDa protein composed of a 348 amino acid extracellular domain (ECD) with three scavenger receptor cysteine-rich (SRCR) domains, a 30 amino acid transmembrane segment, and a 93 amino acid cytoplasmic domain . The extracellular domain is responsible for its interactions with other molecules and cells.
CD5 is widely expressed on the surface of T-cells and a subset of B-cells known as B1 cells. It is also found in certain types of lymphoma cells, such as small lymphocytic lymphoma, hairy cell leukemia, and mantle cell lymphoma .
Biological Functions
CD5 is involved in several key functions within the immune system:
- Regulation of T-cell receptor (TCR) signaling: CD5 modulates the strength and duration of TCR signaling, which is essential for T-cell activation and differentiation.
- B-cell receptor (BCR) signaling: In B1 cells, CD5 plays a role in regulating BCR signaling, which is important for the production of natural antibodies.
- Immune tolerance: CD5 contributes to the maintenance of immune tolerance by preventing overactivation of T-cells and B-cells, thus reducing the risk of autoimmune responses.
Recombinant Human CD5
Recombinant human CD5 is produced using various expression systems, such as HEK293 cells or mouse myeloma cell lines . The recombinant protein typically includes the extracellular domain of CD5 and may have a polyhistidine tag (His tag) at the C-terminus for purification purposes .
The recombinant CD5 protein is used in various research applications, including:
- Functional studies: Investigating the role of CD5 in immune cell signaling and regulation.
- Drug development: Screening for potential therapeutic agents that target CD5 or its signaling pathways.
- Diagnostic assays: Developing assays to detect CD5 expression in different cell types and disease states.
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