CD5 Antibody
Description
Product Specs
Product Science Overview
CD5 belongs to the scavenger receptor cysteine-rich protein superfamily (SRCR) . It plays a crucial role in modulating T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions . The natural ligand for CD5 is CD72 . CD5+ B cells are known to produce polyreactive antibodies, mostly immunoglobulin M (IgM) .
CD5 is primarily expressed on thymocytes, T cells, and B-1 cells . Although mature alpha/beta T cells express high levels of CD5, very few gamma/delta T cells express this antigen . The interaction of CD5 with CD72, gp35-37, T cell receptor (TCR), or B cell receptor (BCR) is involved in T and B cell activation .
CD5 acts as a negative regulator of T-B cell interaction . By setting the threshold level for activation signals, CD5 prevents B cells from activation-induced cell death and maintains tolerance in anergic B cells in vivo . This is crucial for keeping potentially autoreactive cells alive, as these cells are necessary for an effective immune response to some pathogens .
The mouse anti-human CD5 antibody is typically prepared by purifying the antibody through affinity chromatography . The antibody solution is usually stored undiluted between 2°C and 8°C . It is used in various applications such as flow cytometry, immunohistochemistry, and Western blotting .
In flow cytometry, the antibody is used for immunofluorescent staining with flow cytometric analysis . For immunofluorescent staining, it is recommended to use at ≤ 1.0 µg per 10^6 cells in 100 µl volume or 100 µl of whole blood . The reagent should be titrated for optimal performance for each application .
