CD57 FITC Antibody

CD57, also known as HNK-1, NK-1, and Leu-7, is a 110-kilodalton (kDa) glycoprotein expressed on a subset of natural killer (NK) cells and T lymphocytes . It is also found on neural cells and striated muscle . The antigen is a carbohydrate structure associated with myelin-associated glycoprotein . CD57 is not expressed on red blood cells, granulocytes, monocytes, or platelets .

While the exact function of CD57 is not fully understood, it is known to bind to L-selectin, P-selectin, and a fragment of laminin, suggesting a role in cell-matrix interactions . CD57 expression is increased in certain disease states associated with CD4/CD8 imbalances, such as AIDS, autoimmune diseases, viral infections, and allograft transplants .

The CD57 antibody, clone HNK-1, is derived from the hybridization of P3-X63-Ag8.653 mouse myeloma cells with lymph node cells isolated from BALB/c mice immunized with membrane extracts of the HSB-2 T-lymphoblastoid cell line . This monoclonal antibody is of the IgM, κ isotype .

Fluorescein isothiocyanate (FITC) is a widely used fluorescent dye for labeling antibodies. The FITC conjugation allows the CD57 antibody to be used in fluorescence-based detection methods, such as flow cytometry . The antibody solution is typically stored in phosphate-buffered saline with gelatin and 0.1% sodium azide to maintain stability .

CD57 FITC, Mouse Anti-Human is primarily used in flow cytometry to identify and quantify CD57-expressing cells in various research settings . It is also used in immunohistochemistry and other fluorescence-based assays to study the distribution and function of CD57-positive cells .

The antibody should be stored at 2° to 8°C and protected from prolonged exposure to light . Conjugated forms should not be frozen to maintain their functionality .