CD57 Biotin Antibody

The exact function of CD57 is not fully understood. However, its binding to L-selectin, P-selectin, and a fragment of laminin suggests that it may be involved in cell-matrix interactions . CD57 expression is increased in certain disease states associated with CD4/CD8 imbalances, such as AIDS, autoimmune diseases, viral infections, and allograft transplants .

The biotinylated mouse anti-human CD57 antibody is a monoclonal antibody produced by immunizing mice with a membrane extract of the human lymphoblastoid cell line HSB-2 . The antibody is purified by affinity chromatography and conjugated with biotin under optimal conditions . It is typically used for staining applications in flow cytometry, where it is recommended to use ≤1.0 microgram per million cells in a 100 microliter volume .

The antibody solution should be stored undiluted between 2°C and 8°C and should not be frozen . If the antibody is lyophilized, it should be reconstituted with water, mixed gently, and allowed to sit for 30-60 seconds before use . After reconstitution, if not intended for use within a month, it should be aliquoted and stored at -20°C .

The biotinylated mouse anti-human CD57 antibody is used in various research applications, including:

• Flow Cytometry: For the immunofluorescent staining and analysis of CD57 expression on human peripheral blood lymphocytes .
• Western Blotting: For the detection of CD57 in protein samples .