CD4 (26-396) Human, Sf9

CD4 (26-396) Human Recombinant, Sf9
Cat. No.
BT29229
Source
Sf9, Insect cells.
Synonyms
CD4 Molecule, T-Cell Surface Antigen T4/Leu-3, CD4 Antigen (P55), T-Cell Surface Glycoprotein CD4, CD4 Receptor, CD4 Antigen, CD4mut.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

CD4 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 377 amino acids (26-396a.a.) and having a molecular mass of 42.1kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
CD4 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD4 is a cell-surface glycoprotein present on various immune cells, including mature helper T cells, immature thymocytes, monocytes, and macrophages. It is also found on some cytotoxic T cells. Approximately 65% of T cells in the blood are CD4+, indicating the presence of CD4 protein on their membrane. During T cell development, cells initially express both CD4 and CD8 receptors. However, they undergo differentiation in the thymus, leading to specialization and the expression of either CD4 or CD8, but not both.
Description
CD4, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain with a molecular mass of 42.1 kDa. It comprises 377 amino acids (26-396a.a.). On SDS-PAGE, the molecular size appears between 40-57 kDa. This CD4 variant is expressed with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The CD4 protein solution (0.25 mg/ml) consists of phosphate-buffered saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For longer periods, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
CD4 Molecule, T-Cell Surface Antigen T4/Leu-3, CD4 Antigen (P55), T-Cell Surface Glycoprotein CD4, CD4 Receptor, CD4 Antigen, CD4mut.
Source
Sf9, Insect cells.
Amino Acid Sequence
KKVVLGKKGD TVELTCTASQ KKSIQFHWKN SNQIKILGNQ GSFLTKGPSK LNDRADSRRS LWDQGNFPLI IKNLKIEDSD TYICEVEDQK EEVQLLVFGL TANSDTHLLQ GQSLTLTLES PPGSSPSVQC RSPRGKNIQG GKTLSVSQLE LQDSGTWTCT VLQNQKKVEF KIDIVVLAFQ KASSIVYKKE GEQVEFSFPL AFTVEKLTGS GELWWQAERA SSSKSWITFD LKNKEVSVKR VTQDPKLQMG KKLPLHLTLP QALPQYAGSG NLTLALEAKT GKLHQEVNLV VMRATQLQKN LTCEVWGPTS PKLMLSLKLE NKEAKVSKRE KAVWVLNPEA GMWQCLLSDS GQVLLESNIK VLPTWSTPVQ PHHHHHH.

Product Science Overview

CD4 Protein Overview

CD4 is a glycoprotein found on the surface of immune cells such as T-helper cells, monocytes, macrophages, and dendritic cells. It plays a crucial role in the immune system by assisting in the activation of T-cells. CD4 acts as a co-receptor that enhances the interaction between the T-cell receptor (TCR) and the major histocompatibility complex (MHC) class II molecules on antigen-presenting cells.

Structure and Function

The CD4 molecule consists of four immunoglobulin-like domains (D1 to D4). The fragment CD4 (26-396) includes these extracellular domains, which are essential for its interaction with MHC class II molecules. The interaction between CD4 and MHC class II molecules is critical for the activation of T-helper cells, which in turn orchestrate the immune response by signaling other immune cells.

Recombinant Expression in Sf9 Cells

The recombinant CD4 (26-396) protein is expressed in Sf9 insect cells using the baculovirus expression system. This system is widely used for producing recombinant proteins because it allows for proper folding and post-translational modifications, which are essential for the protein’s functionality. The expressed protein is a single, glycosylated polypeptide chain containing 377 amino acids and has a molecular weight of approximately 42.1 kDa .

Applications

Recombinant CD4 (26-396) is used in various research applications, including:

  • Immunology Studies: Understanding the role of CD4 in T-cell activation and immune response.
  • HIV Research: CD4 is the primary receptor for HIV entry into host cells. Studying the interaction between CD4 and HIV can help in developing therapeutic strategies.
  • Drug Development: Screening potential drugs that can modulate CD4 function or its interaction with other molecules.

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