CD4 Human, Active
HEK293 Cells
Greater than 90.0% as determined by SDS-PAGE.
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Description
CD4 Human produced in HEK293 cells is a single, glycosylated polypeptide chain containing 604 amino acids (26-390 a.a.) and having a molecular mass of 67.7kDa. CD4 is expressed with a 239 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
HEK293 Cells
KKVVLGKKGD TVELTCTASQ KKSIQFHWKN SNQIKILGNQ GSFLTKGPSK LNDRADSRRS LWDQGNFPLI IKNLKIEDSD TYICEVEDQK EEVQLLVFGL TANSDTHLLQ GQSLTLTLES PPGSSPSVQC RSPRGKNIQG GKTLSVSQLE LQDSGTWTCT VLQNQKKVEF KIDIVVLAFQ KASSIVYKKE GEQVEFSFPL AFTVEKLTGS GELWWQAERA SSSKSWITFD LKNKEVSVKR VTQDPKLQMG KKLPLHLTLP QALPQYAGSG NLTLALEAKT GKLHQEVNLV VMRATQLQKN LTCEVWGPTS PKLMLSLKLE NKEAKVSKRE KAVWVLNPEA GMWQCLLSDS GQVLLESNIK VLPTWLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH.
Product Science Overview
CD4, also known as T-cell surface glycoprotein CD4 or T-cell surface antigen T4/Leu-3, is a type I transmembrane glycoprotein predominantly expressed on the surface of T-helper cells, monocytes, macrophages, and dendritic cells. It plays a crucial role in the immune response by acting as a co-receptor for the T-cell receptor (TCR) and interacting with major histocompatibility complex (MHC) class II molecules presented by antigen-presenting cells (APCs).
The CD4 molecule consists of four extracellular immunoglobulin-like domains (D1 to D4), a transmembrane region, and a cytoplasmic tail. The extracellular domains are responsible for binding to MHC class II molecules, while the cytoplasmic tail interacts with the Src family tyrosine kinase LCK, which is essential for initiating intracellular signaling pathways.
In the immune response, CD4 enhances the sensitivity of T-cells to antigens presented by MHC class II molecules. This interaction is critical for the activation and differentiation of T-helper cells, which in turn produce cytokines that regulate the immune response. CD4 also plays a role in the differentiation and activation of other immune cells, such as macrophages and natural killer (NK) cells, through TCR/LCK-independent pathways .
Recombinant human CD4 (active) is a laboratory-produced version of the CD4 protein, designed to mimic the natural protein’s structure and function. It is typically expressed in mammalian cell lines, such as Chinese Hamster Ovary (CHO) cells or Human Embryonic Kidney (HEK) 293 cells, to ensure proper folding and post-translational modifications.
Recombinant CD4 is used in various research applications, including studying the immune response, HIV infection mechanisms, and developing therapeutic interventions. It is often utilized in functional assays, such as binding studies with MHC class II molecules, and in structural studies to understand the interaction between CD4 and other proteins .
The recombinant human CD4 protein is valuable in several research areas:
- HIV Research: CD4 is the primary receptor for the Human Immunodeficiency Virus (HIV). Understanding the interaction between CD4 and HIV envelope glycoproteins is crucial for developing vaccines and therapeutic strategies.
- Immunology: CD4’s role in T-cell activation and differentiation makes it a key molecule in studying immune responses, autoimmune diseases, and immunotherapies.
- Structural Biology: Recombinant CD4 is used in crystallography and other structural studies to elucidate the molecular details of its interactions with MHC class II molecules and other proteins.
