CD47 Human, IgG-His
CD47 Human Recombinant, IgG-His Tag
Cat. No.
BT29874
Source
Sf9, Insect cells.
Synonyms
CD47
Molecule, Antigenic Surface Determinant Protein OA3, CD47 Antigen (Rh-Related
Antigen, Integrin-Associated Signal Transducer), Antigen Identified by
Monoclonal, Antibody 1D8, Integrin Associated Protein, Integrin-Associated
Protein, Rh-Related, Antigen, CD47 Glycoprotein, MER6, IAP, Integrin-Associated
Signal Transducer, Leukocyte Surface Antigen CD47, CD47 Antigen, Protein MER6, OA3.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CD47 Human
Recombinant produced in Sf9 Insect cell is a single, non-glycosylated
polypeptide chain containing 362 amino acids (19-141aa.a) and having a
molecular mass of 40.9kDa (Migrates at 40-57kDa on SDS-PAGE under reducing
conditions).
CD47 is fused to a 239 amino acid hIgG-His-tag at C-terminus & purified by proprietary chromatographic techniques.
CD47 is fused to a 239 amino acid hIgG-His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
CD47 is a cell surface receptor that plays a role in various cellular processes, including cell adhesion, immune modulation, and memory formation. It interacts with thrombospondin-1 (THBS1) on platelets, modulates integrin activity, and participates in synaptic plasticity in the hippocampus. CD47 also acts as a receptor for SIRPA, regulating dendritic cell maturation and cytokine production. Moreover, it protects red blood cells from premature elimination and is involved in membrane permeability changes during viral infection.
Description
Recombinant human CD47, expressed in Sf9 insect cells, is a non-glycosylated polypeptide chain consisting of 362 amino acids (residues 19-141). It has a molecular weight of 40.9 kDa and appears between 40-57 kDa on SDS-PAGE under reducing conditions due to glycosylation. The protein is fused to a 239 amino acid hIgG-His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless liquid solution, sterile-filtered.
Formulation
The CD47 protein is provided as a 0.5 mg/ml solution in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is greater than 95.0% as determined by SDS-PAGE analysis.
Biological Activity
The biological activity of recombinant human CD47 is determined by its ability to bind to human SIRP alpha/CD172a (cat# pro-2080) in a functional ELISA assay. The ED50 for this interaction is approximately 100 ng/ml.
Synonyms
CD47
Molecule, Antigenic Surface Determinant Protein OA3, CD47 Antigen (Rh-Related
Antigen, Integrin-Associated Signal Transducer), Antigen Identified by
Monoclonal, Antibody 1D8, Integrin Associated Protein, Integrin-Associated
Protein, Rh-Related, Antigen, CD47 Glycoprotein, MER6, IAP, Integrin-Associated
Signal Transducer, Leukocyte Surface Antigen CD47, CD47 Antigen, Protein MER6, OA3.
Source
Sf9, Insect cells.
Amino Acid Sequence
QLLFNKTKSV
EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL
KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNELEPKSCD KTHTCPPCPA PELLGGPSVF
LFPPKPKDTL MISRTPEVTC VVVDVSHEDP
EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.
EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.
Product Science Overview
Introduction
CD47, also known as Integrin-Associated Protein (IAP), is a transmembrane protein that plays a crucial role in various cellular processes, including cell adhesion, migration, and immune response regulation. The recombinant form of CD47, tagged with IgG and His tags, is widely used in research to study its functions and interactions.
Structure and Expression
CD47 is a glycoprotein that belongs to the immunoglobulin superfamily. It consists of an extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions, and a cytoplasmic tail . The recombinant human CD47 protein is typically expressed in systems such as HEK293 cells or insect cells infected with baculovirus . The His tag at the C-terminus facilitates purification and detection, while the IgG tag enhances stability and solubility.
Biological Functions
CD47 is involved in several critical biological functions:
- Immune Regulation: CD47 interacts with signal regulatory protein alpha (SIRPα) on macrophages, transmitting a “don’t eat me” signal that prevents phagocytosis of healthy cells .
- Cell Adhesion and Migration: CD47 binds to thrombospondin-1 (TSP-1), influencing cell adhesion and migration, which is essential for tissue repair and angiogenesis .
- Apoptosis and Cancer: CD47 expression is often upregulated in cancer cells, helping them evade immune surveillance. Targeting CD47-SIRPα interactions is a promising therapeutic strategy in oncology .
Applications in Research
Recombinant CD47 proteins with IgG-His tags are valuable tools in various research applications:
- Protein-Protein Interaction Studies: The His tag allows for easy immobilization on affinity columns or biosensors, facilitating the study of CD47 interactions with SIRPα and other ligands .
- Functional Assays: Recombinant CD47 can be used in cell-based assays to investigate its role in immune evasion, cell adhesion, and migration .
- Therapeutic Development: CD47 is a target for developing novel cancer immunotherapies. Recombinant CD47 proteins are used in preclinical studies to evaluate the efficacy of potential therapeutic agents .
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