CD46 Human, Sf9
Description
CD46 is fused to 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
CD46 is a glycoprotein that is ubiquitously expressed on all nucleated cells in humans. It consists of four short consensus repeats (SCRs), a serine/threonine/proline (STP)-rich region, a transmembrane domain, and a cytoplasmic tail. The recombinant human CD46 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 268 amino acids (35-313 aa) and has a molecular mass of approximately 32.5 kDa . Due to glycosylation, the molecular size on SDS-PAGE appears at approximately 40-57 kDa .
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Complement Regulation: CD46 acts as a cofactor for factor I-mediated cleavage of C3b and C4b, preventing the formation of the membrane attack complex (MAC) on host cells. This function is vital for protecting host tissues from damage by the complement system .
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Immune Modulation: CD46 is involved in modulating the adaptive immune response. It regulates the production of cytokines such as interferon-gamma (IFN-γ) and interleukin-10 (IL-10) in T helper type 1 (Th1) cells, thereby influencing the balance between pro-inflammatory and anti-inflammatory responses .
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Reproductive Role: CD46 is also implicated in the fusion of spermatozoa with the oocyte during fertilization, highlighting its role beyond immune regulation .
Deficiency or dysfunction of CD46 is associated with various disease conditions, including atypical hemolytic uremic syndrome (aHUS), a disorder characterized by excessive activation of the complement system leading to blood clot formation in small blood vessels . Additionally, CD46 has been identified as a receptor for several pathogens, including measles virus, human herpesvirus-6, and Neisseria gonorrhoeae, which exploit CD46 to gain entry into host cells .
The recombinant human CD46 produced in Sf9 Baculovirus cells is used for research purposes. It is a sterile filtered, colorless solution formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol. The protein is fused to a 6-amino acid His-Tag at the C-terminus and purified using proprietary chromatographic techniques . The recombinant protein is greater than 90% pure as determined by SDS-PAGE .
For optimal stability, the recombinant CD46 should be stored at 4°C if used within 2-4 weeks. For longer storage periods, it should be frozen at -20°C, and it is recommended to add a carrier protein (0.1% HSA or BSA) to prevent degradation. Multiple freeze-thaw cycles should be avoided to maintain protein integrity .
