CD46 Human

CD46 Human Recombinant
Cat. No.
BT29670
Source
E.coli.
Synonyms
AHUS2, MCP, MIC10, TLX, TRA2.10, Membrane cofactor protein, Trophoblast leukocyte common antigen, CD46.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

CD46 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 302 amino acids (35-313 a.a) and having a molecular mass of 33.8kDa. CD46 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Membrane cofactor protein isoform 14 (CD46) is a type I membrane protein that acts as a cofactor for the inactivation of complement components C3b and C4b by serum factor I. This function is located in the complement control protein repeats (CCPs), with CCP1-4 essential for regulation. CD46 is also involved in the fusion of sperm and egg during fertilization. CD46 is commonly found in thymocytes, T cells, B cells, monocytes, granulocytes, NK cells, platelets, endothelial cells, epithelial cells, fibroblasts, placenta, and sperm.
Description
Recombinant human CD46, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 302 amino acids (residues 35-313) and has a molecular weight of 33.8 kDa. The CD46 sequence is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, colorless solution.
Formulation
CD46 protein solution (0.5 mg/ml) in 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 85% by SDS-PAGE analysis.
Synonyms
AHUS2, MCP, MIC10, TLX, TRA2.10, Membrane cofactor protein, Trophoblast leukocyte common antigen, CD46.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSCEEPPTF EAMELIGKPK PYYEIGERVD YKCKKGYFYI PPLATHTICD RNHTWLPVSD DACYRETCPY IRDPLNGQAV PANGTYEFGY QMHFICNEGY YLIGEEILYC ELKGSVAIWS GKPPICEKVL CTPPPKIKNG KHTFSEVEVF EYLDAVTYSC DPAPGPDPFS LIGESTIYCG DNSVWSRAAP ECKVVKCRFP VVENGKQISG FGKKFYYKAT VMFECDKGFY LDGSDTIVCD SNSTWDPPVP KCLKGPRPTY KPPVSNYPGY PKPEEGILDS LD.

Product Science Overview

Structure and Function

CD46 is composed of several domains, including four complement control protein (CCP) repeats, a serine/threonine-rich region, a transmembrane domain, and a cytoplasmic tail. The CCP repeats are essential for its regulatory function, as they serve as cofactors for the inactivation of complement components C3b and C4b by serum factor I . This inactivation prevents the deposition of complement on host tissues, thereby protecting cells from complement-mediated damage .

Role in the Immune System

CD46 has a dual role in the immune system, participating in both innate and adaptive immunity. In the innate immune system, CD46 helps to modulate the activity of macrophages and dendritic cells by suppressing the production of interleukin-12 (IL-12), a pro-inflammatory cytokine . This modulation helps to prevent excessive inflammation and tissue damage.

In the adaptive immune system, CD46 plays a role in regulating T helper type 1 (Th1) immune responses. It influences the balance between the production of interferon-gamma (IFN-γ) and interleukin-10 (IL-10) within Th1 cells . This regulation is important for maintaining immune homeostasis and preventing autoimmune diseases.

Clinical Significance

The importance of CD46 in complement regulation is highlighted by the fact that genetic deficiencies in CD46 can lead to the development of atypical hemolytic-uremic syndrome (aHUS), a disease characterized by uncontrolled complement activation . Additionally, CD46 has been implicated in the development and progression of certain cancers .

Recombinant Human CD46

Recombinant human CD46 is produced using various expression systems, such as HEK293 cells or E. coli, to ensure proper folding and post-translational modifications. The recombinant protein typically includes the extracellular domain of CD46 fused with a polyhistidine tag for purification purposes . The molecular weight of recombinant human CD46 is approximately 34 kDa, but due to glycosylation, it appears as a 55-60 kDa band in SDS-PAGE under reducing conditions .

Recombinant CD46 is used in research to study its role in the immune system, its interactions with pathogens, and its potential therapeutic applications. It is also used in the development of diagnostic assays and therapeutic interventions for diseases related to complement dysregulation .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

CD46 Antibody
CD46, Mouse Anti Human
Cat. No.
BT1162
Source
CD46 Human, Sf9
CD46 Human Recombinant, Sf9
Cat. No.
BT29738
Source
Sf9, Baculovirus cells.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.