sCD40L Human, His Active

soluble CD40 Ligand/TRAP His Tag Active Human Recombinant
Cat. No.
BT209
Source

Escherichia Coli.

Synonyms

CD40 ligand, CD154, CD40L, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

sCD40L Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 169 amino acids (113-261a.a.) and having a molecular mass of 18.3kDa.
sCD40L is fused to a 20 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD40L, also known as CD154, is a glycoprotein found on the surface of T-cells. It plays a crucial role in the immune response by activating B-cells and other immune cells. CD40L interacts with CD40 on B-cells, leading to their proliferation, differentiation, and antibody production. This interaction is essential for a robust immune response against pathogens. Additionally, CD40L can activate monocytes, enhancing their ability to eliminate infected cells and tumors. It also promotes the production of cytokines, such as IFN-gamma, TNF-alpha, and IL2, which further amplify the immune response.
Description
This product consists of the soluble extracellular domain of human CD40L, produced in E. coli. It is a single, non-glycosylated polypeptide chain with a molecular weight of 18.3 kDa. The protein sequence encompasses amino acids 113-261, with an additional 20-amino acid His tag at the N-terminus to facilitate purification. The protein has been purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The sCD40L protein solution (0.5 mg/mL) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 1 mM DTT, and 40% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage to maintain protein stability. Avoid repeated freezing and thawing cycles.
Purity
The purity of this protein is greater than 95% as determined by SDS-PAGE analysis.
Biological Activity
The biological activity of this protein is assessed by its ability to bind to human CD40/TNFRSF5 in a functional ELISA assay.
Synonyms

CD40 ligand, CD154, CD40L, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LCLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

Product Science Overview

Introduction

Soluble CD40 Ligand (sCD40L), also known as CD154, TRAP (TNF-related activation protein), or TNFSF5, is a member of the tumor necrosis factor (TNF) superfamily. It plays a crucial role in the immune system, particularly in the activation and regulation of B cells, T cells, and other immune cells .

Structure and Expression

CD40 Ligand is a type II transmembrane protein predominantly expressed on activated CD4+ T lymphocytes. It can exist in two forms: membrane-bound and soluble. The soluble form is generated through proteolytic cleavage of the membrane-bound form, releasing a portion of the extracellular domain .

The recombinant form of soluble CD40 Ligand with a His tag (a sequence of histidine residues) is produced using various expression systems, such as HEK 293 cells or baculovirus-infected insect cells. The His tag facilitates purification and detection of the protein .

Biological Function

CD40 Ligand interacts with its receptor, CD40, which is expressed on B cells, dendritic cells, macrophages, and other antigen-presenting cells. This interaction is essential for several immune processes, including:

  • B-cell proliferation: Promotes the growth and differentiation of B cells.
  • Immunoglobulin class switching: Facilitates the switch from IgM to other immunoglobulin isotypes, such as IgG, IgA, and IgE.
  • Cytokine production: Induces the production of various cytokines, including IL-4, IL-6, and TNF-α .
Clinical Significance

Defects in the CD40 Ligand gene (CD40LG) can lead to X-linked hyper-IgM syndrome (HIGM1), an immunodeficiency disorder characterized by elevated levels of IgM and reduced levels of other immunoglobulins. Patients with HIGM1 are prone to recurrent bacterial and opportunistic infections .

Additionally, the interaction between CD40 and CD40 Ligand is implicated in various autoimmune diseases, inflammatory conditions, and cancer. As a result, soluble CD40 Ligand is a target for therapeutic interventions aimed at modulating immune responses .

Applications

Recombinant soluble CD40 Ligand with a His tag is widely used in research and clinical applications, including:

  • Immunology studies: Investigating the role of CD40-CD40L interactions in immune responses.
  • Drug development: Screening for potential therapeutic agents that target CD40-CD40L interactions.
  • Diagnostic assays: Developing assays to measure soluble CD40 Ligand levels in biological samples .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

sCD40L Human
Soluble CD-40 Ligand/TRAP Human Recombinant
Cat. No.
BT91
Source
Escherichia Coli.
sCD40L Human, His
soluble CD40 Ligand/TRAP Human Recombinant, His Tag
Cat. No.
BT134
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.