CD36 Human
Platelet glycoprotein 4 isoform 1, BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3, Glycoprotein IIIb, GPIIIB, Leukocyte differentiation antigen CD36, PAS-4, Platelet collagen receptor, Platelet glycoprotein IV, Thrombospondin receptor.
Greater than 85.0% as determined by SDS-PAGE.
Description
CD36 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 652 amino acids (30-439a.a.) and having a molecular mass of 73.9kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
CD36 is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Platelet glycoprotein 4 isoform 1, BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3, Glycoprotein IIIb, GPIIIB, Leukocyte differentiation antigen CD36, PAS-4, Platelet collagen receptor, Platelet glycoprotein IV, Thrombospondin receptor.
ADLGDLLIQK TIKKQVVLEE GTIAFKNWVK TGTEVYRQFW IFDVQNPQEV MMNSSNIQVK QRGPYTYRVR FLAKENVTQD AEDNTVSFLQ PNGAIFEPSL SVGTEADNFT VLNLAVAAAS HIYQNQFVQM ILNSLINKSK SSMFQVRTLR ELLWGYRDPF LSLVPYPVTT TVGLFYPYNN
TADGVYKVFN GKDNISKVAI IDTYKGKRNL SYWESHCDMI NGTDAASFPP FVEKSQVLQF FSSDICRSIY AVFESDVNLK GIPVYRFVLP SKAFASPVEN PDNYCFCTEK IISKNCTSYG VLDISKCKEG RPVYISLPHF LYASPDVSEP IDGLNPNEEE HRTYLDIEPI TGFTLQFAKR
LQVNLLVKPS EKIQVLKNLK RNYIVPILWL NETGTIGDEK ANMFRSQVTG KINLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG
QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.
Product Science Overview
CD36 is a single-chain protein consisting of 472 amino acid residues with both N- and C-terminal cytoplasmic tails and an extracellular loop . It is expressed on a variety of cell types, including platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells, and some skin microdermal endothelial cells . The protein is localized to the Golgi apparatus and is found in the cytoplasm of endothelial cells, liver sinusoids, adipocytes, thrombocytes, megakaryocytes, skeletal and cardiac muscle cells .
CD36 serves as a multiligand pattern recognition receptor that interacts with a wide range of structurally dissimilar ligands. These include long-chain fatty acids (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high-density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils, collagens I and IV, and Plasmodium falciparum-infected erythrocytes . Its primary functions include:
- Fatty Acid Metabolism: CD36 plays a crucial role in the uptake and transport of long-chain fatty acids, contributing to muscle lipid uptake, adipose energy storage, and gut fat absorption .
- Immune Response: It acts as a receptor for various ligands involved in the immune response, including oxidized lipids and apoptotic cells .
- Inflammation: CD36 is involved in the inflammatory response by recognizing and binding to oxidized lipids and other inflammatory molecules .
Recombinant human CD36 is produced using various expression systems, including insect cells (Spodoptera frugiperda, Sf21) and human embryonic kidney (HEK) 293 cells . The recombinant protein is often used in research to study its binding properties and interactions with other molecules. For example, recombinant human CD36/Fc chimera has been shown to bind to thrombospondin-2 in a functional ELISA .
CD36 is associated with several diseases, including cardiovascular diseases, metabolic disorders, and cancer . Its role in fatty acid metabolism and inflammation makes it a potential target for therapeutic interventions in these conditions. Additionally, CD36 is being investigated as a potential biomarker for various diseases due to its involvement in multiple physiological processes .
