CD276 Human
CD276 Molecule, CD276 Antigen, Costimulatory Molecule, B7 Homolog 3, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2.
Greater than 90.0% as determined by SDS-PAGE.
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Description
CD276 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 446 amino acids (29-466a.a.) and having a molecular mass of 48.1kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). CD276 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD276 is a member of the B7 family and plays a critical role in immune regulation. This transmembrane glycoprotein is found on T cells and functions as a costimulatory molecule, enhancing T cell activation and IFN-gamma production. It also contributes to DNA integrity by upregulating BRCC3 expression, which protects against 5-Fu-induced damage. CD276 is linked to the progression of Non-Small Cell Lung Cancer (NSCLC), serving as a potential biomarker for NSCLC-derived malignant pleural effusions (MPEs). This protein also inhibits the destruction of tumor cells by natural killer cells and aids in detecting neuroblastoma cells. Beyond cancer, CD276 is implicated in both acute and chronic transplant rejection and helps regulate lymphocyte activity in mucosal tissues. During pregnancy, it plays a crucial role in maintaining a balanced immune environment for the placenta and fetus.
Recombinant human CD276, produced in Sf9 insect cells using a baculovirus expression system, is a single-chain glycoprotein. It consists of 446 amino acids (residues 29-466), with an 8 amino acid His tag at the C-terminus, and has a molecular weight of 48.1 kDa. Note that on SDS-PAGE, it appears as a band at approximately 50-70 kDa due to glycosylation. The protein is purified using proprietary chromatographic techniques to ensure high purity.
The CD276 protein is supplied in a solution containing 0.5 mg/ml of the protein in a phosphate-buffered saline solution (pH 7.4) with 10% glycerol added.
The purity of this CD276 protein is greater than 90%, as determined by SDS-PAGE analysis.
CD276 Molecule, CD276 Antigen, Costimulatory Molecule, B7 Homolog 3, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2.
LEVQVPEDPV VALVGTDATL CCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FAEGQDQGSA YANRTALFPD LLAQGNASLR LQRVRVADEG SFTCFVSIRD FGSAAVSLQV AAPYSKPSMT LEPNKDLRPG DTVTITCSSY QGYPEAEVFW QDGQGVPLTG NVTTSQMANE QGLFDVHSIL RVVLGANGTY SCLVRNPVLQ QDAHSSVTIT PQRSPTGAVE VQVPEDPVVA LVGTDATLRC SFSPEPGFSL AQLNLIWQLT DTKQLVHSFT EGRDQGSAYA NRTALFPDLL AQGNASLRLQ RVRVADEGSF TCFVSIRDFG SAAVSLQVAA PYSKPSMTLE PNKDLRPGDT VTITCSSYRG YPEAEVFWQD GQGVPLTGNV TTSQMANEQG LFDVHSVLRV VLGANGTYSC LVRNPVLQQD AHGSVTITGQ PMTFPPEALE HHHHHH.
Product Science Overview
CD276 is a type I transmembrane protein with signature IgV and IgG regions in its extracellular domains . It shares 20-27% amino acid identity with other B7 family members . The human recombinant form of CD276 is typically expressed in HEK293 cells and has a calculated molecular weight of approximately 47.3 kDa, although it can appear as 60-80 kDa on SDS-PAGE due to glycosylation .
CD276 is expressed on various immune cells, including T cells, natural killer cells, and antigen-presenting cells. It is also found on some non-immune cells such as osteoblasts, fibroblasts, and epithelial cells . High expression of CD276 in tumor vasculature has been correlated with poor survival in cancer patients, suggesting its role in tumor cell migration .
CD276 has a dual role in the immune system, acting as both a costimulatory and coinhibitory molecule . It is involved in the activation of T lymphocytes and the regulation of murine bone formation . Additionally, CD276 has been shown to inhibit the function of T cells, making it a potential target for immunotherapy in various types of cancer .
Recent studies have highlighted the clinical prognostic value of CD276 in glioblastoma and other major cancer types . High expression of CD276 is often associated with poorer survival and prognosis in many cancers . It is also closely associated with T cell infiltration, immune checkpoint genes, and immunoregulatory interactions between lymphoid and non-lymphoid cells .
The recombinant form of CD276 is used extensively in research to study its role in immune regulation and cancer. It is also being explored as a potential target for cancer immunotherapy . CD276’s ability to upregulate BRCC3 expression and prevent DNA defects caused by chemotherapeutic agents like 5-Fu further underscores its significance in cancer research .
In conclusion, CD276 (Human Recombinant) is a vital protein with significant implications in immune regulation and cancer therapy. Its dual role in the immune system and its association with poor prognosis in various cancers make it a promising target for future research and therapeutic interventions.
