CD276 Mouse
CD276 antigen, Cd276, 6030411F23Rik, AU016588, B7h3, B7RP-2, B7 homolog 3, B7-H3.
Greater than 90.0% as determined by SDS-PAGE.
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Description
CD276 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 226 amino acids (29-248a.a.) and having a molecular mass of 24.7kDa.
CD276 is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
CD276, a member of the B7 family, is an immunoregulatory transmembrane glycoprotein found on T cells. It functions as a costimulatory molecule, enhancing T cell activation and IFN-gamma production. Additionally, CD276 increases BRCC3 expression, which protects against DNA damage induced by 5-Fu. There is a correlation between CD276 levels and the TNM stage in NSCLC, suggesting its potential as a biomarker for NSCLC-derived MPEs. CD276 is involved in inhibiting natural-killer cell-mediated lysis in tumor cells and serves as a marker for identifying neuroblastoma cells. It plays a role in both acute and chronic transplant rejection, as well as in regulating lymphocyte activity at mucosal surfaces. Furthermore, CD276 contributes to establishing a suitable immunological environment for the placenta and fetus during pregnancy.
Produced in Sf9 Baculovirus cells, CD276 is a single, glycosylated polypeptide chain. It consists of 226 amino acids (29-248a.a.), resulting in a molecular weight of 24.7kDa. This protein includes a C-terminal 6 amino acid His-Tag and undergoes purification using proprietary chromatographic methods.
This solution contains CD276 protein at a concentration of 0.25mg/ml. It is formulated in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
For short-term storage (up to 4 weeks), the unopened vial should be stored at 4°C. For longer storage, it is recommended to freeze the protein at -20°C. The addition of a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. Repeated freezing and thawing of the protein should be avoided.
The purity of this protein is determined to be greater than 90% using SDS-PAGE analysis.
CD276 antigen, Cd276, 6030411F23Rik, AU016588, B7h3, B7RP-2, B7 homolog 3, B7-H3.
VEVQVSEDPV VALVDTDATL RCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FTEGRDQGSA
YSNRTALFPD LLVQGNASLR LQRVRVTDEG SYTCFVSIQD FDSAAVSLQV AAPYSKPSMT
LEPNKDLRPG NMVTITCSSY QGYPEAEVFW KDGQGVPLTG NVTTSQMANE RGLFDVHSVL
RVVLGANGTY SCLVRNPVLQ QDAHGSVTIT GQPLTFPPEA HHHHHH
Product Science Overview
CD276, also known as B7-H3, is a member of the B7 family of immune regulatory ligands. It plays a crucial role in the regulation of T cell activation and immune responses. This protein is of significant interest in the field of immunology and cancer research due to its involvement in various immune processes and its potential as a therapeutic target.
CD276 is a type I transmembrane protein that contains two extracellular immunoglobulin (Ig)-like domains: one IgV-like and one IgC-like domain . In mice, the extracellular portion of CD276 is composed of these two Ig domains, whereas in humans, it has four Ig domains due to exon duplication . The protein also includes a transmembrane domain and a short intracellular domain .
CD276 is known to modulate immune responses by either promoting or inhibiting T-cell responses, depending on the context . It is involved in the attenuation of peripheral immune responses through co-inhibition, playing an important role in adaptive immune responses . CD276 has been shown to either promote or inhibit T-cell responses in various experimental systems, indicating its complex role in immune regulation.
CD276 is highly expressed in various types of cancer and is often associated with poor prognosis . Its expression is closely linked to T cell infiltration, immune checkpoint genes, and immunoregulatory interactions between lymphoid and non-lymphoid cells . This makes CD276 a promising target for immunotherapy in cancer treatment. Studies have shown that CD276 can inhibit the function of T cells, making it a potential target for therapies aimed at enhancing anti-tumor immune responses .
Recombinant mouse CD276 is produced using DNA sequences encoding the mouse CD276 protein, which is then expressed in host cells such as HEK293 cells . The recombinant protein is often tagged with polyhistidine for purification purposes and may be biotinylated for use in various assays . The recombinant mouse CD276 protein is used in research to study its function, interactions, and potential as a therapeutic target.
Recombinant mouse CD276 is widely used in immunological research to study its role in immune regulation and its potential as a therapeutic target in cancer. It is used in various assays to investigate its interactions with other immune molecules and its effects on T-cell responses. The protein’s high biological activity and stability make it a valuable tool for researchers studying immune responses and developing new immunotherapies .
