CD207 Human

CD207 Human Recombinant
Cat. No.
BT26632
Source
Escherichia Coli.
Synonyms
C-type lectin domain family 4 member K, CLEC4K, Langerin, CD207.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CD207 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 287 amino acids (65-328 a.a) and having a molecular mass of 32.2kDa.
CD207 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD207, also known as C-type lectin domain family 4 member K, is a protein primarily found on Langerhans cells, which are immature dendritic cells located in the epidermis and mucosa. Its expression extends to other dendritic cell types like dermal CD103+ DCs and splenic CD8+ DCs. Within Langerhans cells, CD207 is found in Birbeck granules, unique organelles characterized by their zippered membrane structures. As a C-type lectin, CD207 exhibits mannose-binding properties. This binding is believed to trigger the internalization of antigens into Birbeck granules, suggesting a role for CD207 in a non-classical antigen processing pathway.
Description
Recombinant human CD207, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 287 amino acids (residues 65-328), resulting in a molecular weight of 32.2 kDa. The protein features a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The CD207 protein solution is provided at a concentration of 1 mg/ml in a buffer containing 20 mM Tris (pH 8.0) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to store the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the CD207 protein is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
C-type lectin domain family 4 member K, CLEC4K, Langerin, CD207.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSPRFMGTI SDVKTNVQLL KGRVDNISTL DSEIKKNSDG MEAAGVQIQM VNESLGYVRS QFLKLKTSVE KANAQIQILT RSWEEVSTLN AQIPELKSDL EKASALNTKI RALQGSLENM SKLLKRQNDI LQVVSQGWKY FKGNFYYFSL IPKTWYSAEQ FCVSRNSHLT SVTSESEQEF LYKTAGGLIY WIGLTKAGME GDWSWVDDTP FNKVQSARFW IPGEPNNAGN NEHCGNIKAP SLQAWNDAPC DKTFLFICKR PYVPSEP.

Product Science Overview

Structure and Function

Langerin/CD207 is characterized by its ability to form “tennis racket”-shaped Birbeck granules within Langerhans cells. These granules are unique endosomal recycling compartments that play a crucial role in the immune response . The protein has a molecular mass of approximately 31 kDa, but it can appear as 35-40 kDa under reducing conditions in SDS-PAGE analysis .

Role in Immune System

Langerin/CD207 is involved in the recognition and internalization of pathogens. It binds to mannose-containing carbohydrates on the surface of pathogens, facilitating their uptake and processing by Langerhans cells. This process is essential for the initiation of the immune response and the presentation of antigens to T cells .

Recombinant Human Langerin/CD207

Recombinant forms of Langerin/CD207 are produced using various expression systems, such as mouse myeloma cell lines (NS0) or Chinese Hamster Ovary (CHO) cells. These recombinant proteins are often tagged with His-tags to facilitate purification and detection .

Applications:

  • Research: Recombinant Langerin/CD207 is widely used in immunological research to study the function and behavior of Langerhans cells.
  • Diagnostics: It serves as a diagnostic marker for identifying Langerhans cells in tissue samples.
  • Therapeutics: Potential therapeutic applications are being explored, particularly in the context of enhancing immune responses against pathogens and tumors.
Stability and Storage

Recombinant Human Langerin/CD207 proteins are typically lyophilized and require reconstitution in sterile PBS. They should be stored at -20 to -70°C to maintain stability and avoid repeated freeze-thaw cycles .

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