ALCAM (CD166) Mouse
Sf9, Baculovirus cells.
Activated leukocyte cell adhesion molecule, ALCAM, Alcam, CD166 antigen, CD166 antigen isoform1, CD166, Protein DM-GRASP, AI853494 Protein, MuSC, SC1, BEN.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
ALCAM mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 739 amino acids (28-527a.a.) and having a molecular mass of 83.1kDa.
ALCAM is expressed with a 239 amino acid hIgG-His-Tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD166 antigen isoform 2, also known as ALCAM, is a transmembrane glycoprotein classified under the immunoglobulin superfamily. This protein is found on various cells, including thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and dendritic cells derived from monocytes. The interaction between CD166 and CD6 plays a crucial role in T cell development and regulation, and facilitates the binding of T cells and B cells to activated leukocytes.
Produced in Sf9 Baculovirus cells, the mouse ALCAM protein is a single, glycosylated polypeptide chain. It comprises 739 amino acids (28-527a.a.) and has a molecular weight of 83.1kDa. The protein is expressed with a C-terminal 239 amino acid hIgG-His-Tag and purified using proprietary chromatographic techniques.
Sterile filtered, colorless solution.
The ALCAM protein solution is provided at a concentration of 0.25mg/ml and contains 50% glycerol in Phosphate-Buffered Saline (pH 7.4).
For short-term storage (2-4 weeks), the solution can be stored at 4°C. For extended periods, store frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeated freezing and thawing cycles.
The purity is determined to be greater than 90.0% by SDS-PAGE analysis.
The biological activity is evaluated based on the protein's ability to inhibit the adhesion of Jurkat human acute T cell leukemia cells to immobilized Recombinant Human CD6. The addition of 1 µg/ml of mouse ALCAM bound to CD6 results in over 50% inhibition of cell adhesion.
Activated leukocyte cell adhesion molecule, ALCAM, Alcam, CD166 antigen, CD166 antigen isoform1, CD166, Protein DM-GRASP, AI853494 Protein, MuSC, SC1, BEN.
Sf9, Baculovirus cells.
WYTVNSAYGD TIVMPCRLDV PQNLMFGKWK YEKPDGSPVF IAFRSSTKKS VQYDDVPEYK DRLSLSENYT LSIANAKISD EKRFVCMLVT EDNVFEAPTL VKVFKQPSKP EIVNKAPFLE TDQLKKLGDC ISRDSYPDGN ITWYRNGKVL QPVEGEVAIL FKKEIDPGTQ LYTVTSSLEY KTTRSDIQMP FTCSVTYYGP SGQKTIYSEQ EIFDIYYPTE QVTIQVLPPK NAIKEGDNIT
LQCLGNGNPP PEEFMFYLPG QPEGIRSSNT YTLTDVRRNA TGDYKCSLID KRNMAASTTI TVHYLDLSLN PSGEVTKQIG DTLPVSCTIS ASRNATVVWM KDNIRLRSSP SFSSLHYQDA GNYVCETALQ EVEGLKKRES LTLIVEGKPQ IKMTKKTDPS GLSKTIICHV EGFPKPAIHW TITGSGSVIN QTEESPYING RYYSKIIISP EENVTLTCTA ENQLERTVNS LNVSAISIPE
HDEADDISDE NREKVNDQAK LEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK HHHHHH
Product Science Overview
CD166 is characterized by its five immunoglobulin-like domains in the extracellular region, which facilitate its role in cell-cell adhesion . It engages in both homotypic (CD166-CD166) and heterotypic (CD166-other molecules) interactions, contributing to various cellular behaviors such as axonal guidance, leukocyte homing, and cancer metastasis .
CD166 has been implicated in the progression of several cancers, including multiple myeloma and breast cancer. In multiple myeloma, CD166 expression is associated with enhanced homing of cancer cells to the bone marrow, promoting osteolytic disease and tumor progression . Similarly, high expression of CD166 in breast cancer is linked to tumor progression and poor prognosis .
Research on CD166 continues to uncover its diverse roles in health and disease. Studies have shown that CD166 can function as a cell surface sensor for cell density, controlling the transition between local cell proliferation and tissue invasion in melanoma progression . Additionally, CD166’s involvement in immune responses and tissue development makes it a valuable target for therapeutic interventions beyond oncology.
