CD163 Human
CD163 Molecule, Hemoglobin Scavenger Receptor, CD163 Antigen, M130, Scavenger Receptor Cysteine-Rich Type 1 Protein M130, Macrophage-Associated Antigen, SCARI1, MM130,CD163.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD163 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 1015 amino acids (42-1050a.a.) and having a molecular mass of 109.8kDa. (Molecular size on SDS-PAGE will appear at approximately 100kDa). CD163 is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD163 Molecule, Hemoglobin Scavenger Receptor, CD163 Antigen, M130, Scavenger Receptor Cysteine-Rich Type 1 Protein M130, Macrophage-Associated Antigen, SCARI1, MM130,CD163.
SSLGGTDKEL RLVDGENKCS GRVEVKVQEE WGTVCNNGWS MEAVSVICNQ LGCPTAIKAP GWANSSAGSG RIWMDHVSCR GNESALWDCK HDGWGKHSNC THQQDAGVTC SDGSNLEMRL TRGGNMCSGR IEIKFQGRWG TVCDDNFNID HASVICRQLE CGSAVSFSGS SNFGEGSGPI WFDDLICNGN ESALWNCKHQ GWGKHNCDHA EDAGVICSKG ADLSLRLVDG VTECSGRLEV RFQGEWGTIC DDGWDSYDAA VACKQLGCPT AVTAIGRVNA SKGFGHIWLD SVSCQGHEPA IWQCKHHEWG KHYCNHNEDA GVTCSDGSDL ELRLRGGGSR CAGTVEVEIQ RLLGKVCDRG WGLKEADVVC RQLGCGSALK TSYQVYSKIQ ATNTWLFLSS CNGNETSLWD CKNWQWGGLT CDHYEEAKIT CSAHREPRLV GGDIPCSGRV EVKHGDTWGS ICDSDFSLEA ASVLCRELQC GTVVSILGGA HFGEGNGQIW AEEFQCEGHE SHLSLCPVAP RPEGTCSHSR DVGVVCSRYT EIRLVNGKTP CEGRVELKTL GAWGSLCNSH WDIEDAHVLC QQLKCGVALS TPGGARFGKG NGQIWRHMFH CTGTEQHMGD CPVTALGASL CPSEQVASVI CSGNQSQTLS SCNSSSLGPT RPTIPEESAV ACIESGQLRL VNGGGRCAGR VEIYHEGSWG TICDDSWDLS DAHVVCRQLG CGEAINATGS AHFGEGTGPI WLDEMKCNGK ESRIWQCHSH GWGQQNCRHK EDAGVICSEF MSLRLTSEAS REACAGRLEV FYNGAWGTVG KSSMSETTVG VVCRQLGCAD KGKINPASLD KAMSIPMWVD NVQCPKGPDT LWQCPSSPWE KRLASPSEET WITCDNKIRL QEGPTSCSGR VEIWHGGSWG TVCDDSWDLD DAQVVCQQLG CGPALKAFKE AEFGQGTGPI WLNEVKCKGN ESSLWDCPAR RWGHSECGHK EDAAVNCTDI SVQKTPQKAT TGRSSRQSSH HHHHH
Product Science Overview
CD163 is a 130-160 kDa protein with a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) containing 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39-84 aa cytoplasmic region . The protein is expressed on the surface of macrophages and is involved in various biological processes, including endocytosis, pathogen binding, and signal transduction .
CD163 is primarily expressed on the surface of macrophages, particularly M2 type macrophages, which are associated with anti-inflammatory responses and tissue repair . The protein mediates the clearance of Hb-Hp complexes, thereby preventing oxidative damage and inflammation caused by free hemoglobin . Additionally, CD163 can bind to tumor necrosis factor-like weak inducer of apoptosis (TWEAK), playing a role in regulating cytokine production by macrophages .
The primary function of CD163 is to bind and internalize Hb-Hp complexes, which are then degraded in lysosomes . This process helps to prevent the accumulation of free hemoglobin in the bloodstream, which can cause oxidative stress and damage to tissues . CD163 also plays a role in the immune response by binding to pathogens and facilitating their clearance by macrophages .
CD163 expression is regulated by various factors, including inflammatory cytokines and stress signals . In response to inflammatory stimuli, the extracellular domain of CD163 can be cleaved by metalloproteinases, resulting in the release of a soluble form of CD163 (sCD163) into the bloodstream . Elevated levels of sCD163 are associated with various inflammatory diseases, indicating its potential as a biomarker for inflammation .
Recombinant human CD163 is produced using mammalian cell expression systems, such as mouse myeloma cell lines . The recombinant protein retains its ability to bind Hb-Hp complexes and is used in various research applications, including studies on macrophage function, inflammation, and immune response .
