CCNB2 Human
Cyclin-B2 Human Recombinant
Cat. No.
BT2200
Source
E.coli.
Synonyms
G2/mitotic-specific cyclin-B2, HsT17299, cyclin B2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CCNB2 Human Recombinant produced in E. coli is a single polypeptide chain containing 422 amino acids (1-398) and having a molecular mass of 47.9 kDa.
CCNB2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CCNB2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
CCNB2, a member of the cyclin family, plays a crucial role in regulating the cell cycle, specifically at the G2/M (mitosis) transition. It interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex known as maturation promoting factor (MPF).
Description
Recombinant human CCNB2, produced in E. coli, is a single polypeptide chain consisting of 422 amino acids (residues 1-398) with a molecular weight of 47.9 kDa. This protein features a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The CCNB2 solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 5 mM DTT, and 50% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the CCNB2 protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
G2/mitotic-specific cyclin-B2, HsT17299, cyclin B2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMALLRR PTVSSDLENI DTGVNSKVKS HVTIRRTVLE EIGNRVTTRA AQVAKKAQNT KVPVQPTKTT NVNKQLKPTA SVKPVQMEKL APKGPSPTPE DVSMKEENLC QAFSDALLCK IEDIDNEDWE NPQLCSDYVK DIYQYLRQLE VLQSINPHFL DGRDINGRMR AILVDWLVQV HSKFRLLQET LYMCVGIMDR FLQVQPVSRK KLQLVGITAL LLASKYEEMF SPNIEDFVYI TDNAYTSSQI REMETLILKE LKFELGRPLP LHFLRRASKA GEVDVEQHTL AKYLMELTLI DYDMVHYHPS KVAAAASCLS QKVLGQGKWN LKQQYYTGYT ENEVLEVMQH MAKNVVKVNE NLTKFIAIKN KYASSKLLKI SMIPQLNSKA VKDLASPLIG RSc
Product Science Overview
Structure and Expression
Cyclin-B2 is a protein that in humans is encoded by the CCNB2 gene located on chromosome 15 (15q22.2) . The recombinant form of Cyclin-B2 is often produced in Escherichia coli and purified using conventional chromatography techniques . The recombinant protein typically includes an N-terminal His-tag to facilitate purification and detection .
Function
Cyclin-B2 associates with CDK1 (Cyclin-dependent kinase 1) to form a serine/threonine kinase holoenzyme complex known as the maturation promoting factor (MPF) . This complex is pivotal for the progression of cells from the G2 phase to the M phase of the cell cycle . Cyclin-B2 is primarily associated with the Golgi region, differentiating it from Cyclin-B1, which co-localizes with microtubules .
Biological Significance
Cyclin-B2 is involved in several key cellular processes:
- Cell Cycle Regulation: It regulates the G2/M transition, ensuring proper cell division .
- Protein Interactions: Cyclin-B2 interacts with transforming growth factor beta receptor 2 (TGFβR2), playing a role in TGFβ-mediated cell cycle control .
- Subcellular Localization: Its association with the Golgi apparatus suggests a role in Golgi-related functions during cell division .
Applications
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