CCNB1 Human

Cyclin-B1 forms a complex with CDK1 (also known as CDC2), and this complex is essential for the initiation of mitosis. The activation of the Cyclin B1-CDK1 complex leads to various cellular processes, including chromosome condensation, nuclear envelope breakdown, and spindle formation . The levels of Cyclin-B1 fluctuate throughout the cell cycle, peaking during the G2/M transition and rapidly degrading as the cell exits mitosis .

Recombinant Cyclin-B1 is produced using recombinant DNA technology, where the gene encoding Cyclin-B1 is cloned and expressed in a suitable host system, such as bacteria or insect cells. This allows for the production of large quantities of Cyclin-B1 protein, which can be used for various research and therapeutic purposes .

Recombinant Cyclin-B1 is widely used in cell cycle studies to understand its role in cell division and its regulation. It is also used in biochemical assays to study the interaction between Cyclin-B1 and CDK1, as well as other regulatory proteins involved in cell cycle control . Additionally, Cyclin-B1 is a target for cancer research, as its overexpression has been observed in various cancers, including breast, prostate, and non-small cell lung cancers .

Quantification of Cyclin-B1 levels in cells is typically performed using techniques such as Western blotting and flow cytometry. These methods allow researchers to measure the amount of Cyclin-B1 protein and its distribution within the cell cycle . Studies have shown that Cyclin-B1 expression begins in the G1 phase, increases non-linearly, and peaks in the G2 phase, indicating tight quantitative control of its expression .