CBX3 Human

Chromobox Homolog 3 Human Recombinant
Cat. No.
BT8283
Source
E.coli.
Synonyms
Chromobox homolog 3 (Drosophila HP1 gamma), Heterochromatin protein 1 homolog gamma, Modifier 2 protein, HP1 gamma homolog, HECH, HP1Hs-gamma.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CBX3 Human Recombinant produced in E. coli is a single polypeptide chain containing 207 amino acids (1-183) and having a molecular mass of 23.4kDa.
CBX3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CBX3 is a protein involved in transcriptional silencing within heterochromatin-like complexes. It achieves this by binding to histone H3 tails that have been methylated at the 'Lys-9' position, leading to epigenetic repression. Furthermore, CBX3 contributes to the formation of functional kinetochores through interactions with proteins belonging to the MIS12 complex.
Description
Recombinant human CBX3, produced in E. coli, is a single polypeptide chain consisting of 207 amino acids (residues 1-183) with a molecular weight of 23.4 kDa. This protein includes a 24 amino acid His-tag fused to the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The CBX3 solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is advisable for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of CBX3 is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
Chromobox homolog 3 (Drosophila HP1 gamma), Heterochromatin protein 1 homolog gamma, Modifier 2 protein, HP1 gamma homolog, HECH, HP1Hs-gamma.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMASNKT TLQKMGKKQN GKSKKVEEAE PEEFVVEKVL DRRVVNGKVE YFLKWKGFTD ADNTWEPEEN LDCPELIEAF LNSQKAGKEK DGTKRKSLSD SESDDSKSKK KRDAADKPRG FARGLDPERI IGATDSSGEL MFLMKWKDSD EADLVLAKEA NMKCPQIVIA FYEERLTWHS CPEDEAQ.

Product Science Overview

Structure and Function

CBX3 is involved in transcriptional silencing within heterochromatin-like complexes. It recognizes and binds to histone H3 tails that are methylated at lysine 9 (H3K9me), leading to epigenetic repression . This binding is essential for the formation of functional kinetochores through interaction with the MIS12 complex proteins .

Biological Role

CBX3 plays a significant role in various cellular processes, including:

  • Transcriptional Repression: By binding to methylated histone H3 tails, CBX3 helps in silencing gene expression in heterochromatin regions .
  • Epigenetic Modification: It is involved in the modification of chromatin structure, thereby influencing gene expression patterns .
  • Cell Differentiation: CBX3 is crucial for the differentiation of cells, impacting their development and function .
Clinical Significance

Recent studies have highlighted the importance of CBX3 in cancer biology. Increased expression of CBX3 has been associated with tumorigenesis in various cancers . For instance, high levels of CBX3 expression correlate with adverse prognosis in patients with certain types of cancer . Additionally, CBX3’s role in the tumor microenvironment, including immune infiltration and interaction with cancer-associated fibroblasts, has been a focus of research .

Recombinant Human CBX3

Recombinant human CBX3 is a form of the protein that is produced using recombinant DNA technology. This involves inserting the CBX3 gene into a suitable expression system, such as E. coli, to produce the protein in large quantities . The recombinant protein is often tagged with a His-tag to facilitate purification and is used in various research applications to study its function and interactions .

Applications in Research

Recombinant CBX3 is widely used in research to:

  • Study Protein-Protein Interactions: Understanding how CBX3 interacts with other proteins can provide insights into its role in chromatin organization and gene regulation .
  • Investigate Epigenetic Mechanisms: Researchers use recombinant CBX3 to explore how epigenetic modifications influence gene expression and cellular function .
  • Develop Cancer Therapies: Given its role in tumorigenesis, CBX3 is a potential target for developing new cancer treatments .

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