CBX1 Human
Chromobox Homolog 1 Human Recombinant
Cat. No.
BT8200
Source
Escherichia Coli.
Synonyms
Chromobox homolog 1, CBX, M31, HP1-BETA, HP1Hs-beta, MOD1, Heterochromatin protein 1 homolog beta, Modifier 1 protein, p25beta, Chromobox homolog 1 (Drosophila HP1 beta), Heterochromatin protein p25.
Appearance
CBX1 is supplied as a sterile filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CBX1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 205 amino acids (1-185) and having a molecular mass of 23.6kDa (molecular weight on SDS-PAGE will appear higher).
The CBX1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
CBX1, a member of the heterochromatin protein family, is an enriched heterochromatin. It plays a crucial role in epigenetic repression by identifying and binding to histone H3 tails methylated at 'Lys-9' at centromeres. Its interaction with lamin B receptor (LBR) can influence the relationship between heterochromatin and the inner nuclear membrane. CBX1 is essential for the epigenetic regulation of chromatin structure and gene expression.
Description
Recombinant human CBX1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 205 amino acids (1-185) with a molecular mass of 23.6 kDa. While its actual molecular weight is 23.6 kDa, it may appear higher on SDS-PAGE due to the presence of a 20 amino acid His-Tag fused at the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
CBX1 is provided as a clear solution that has been sterilized through filtration.
Formulation
CBX1 protein is supplied at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8), 0.1M NaCl, 2mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), CBX1 can be stored at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. To ensure long-term stability, adding a carrier protein like HSA or BSA (0.1%) is advisable. Avoid repeated freezing and thawing of the protein.
Purity
The purity of CBX1 is determined to be greater than 85% using SDS-PAGE analysis.
Synonyms
Chromobox homolog 1, CBX, M31, HP1-BETA, HP1Hs-beta, MOD1, Heterochromatin protein 1 homolog beta, Modifier 1 protein, p25beta, Chromobox homolog 1 (Drosophila HP1 beta), Heterochromatin protein p25.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGKKQNKKKV EEVLEEEEEE YVVEKVLDRR VVKGKVEYLL KWKGFSDEDN TWEPEENLDC PDLIAEFLQS QKTAHETDKS EGGKRKADSD SEDKGEESKP KKKKEESEKP RGFARGLEPE RIIGATDSSG ELMFLMKWKN SDEADLVPAK EANVKCPQVV ISFYEERLTW HSYPSEDDDK KDDKN
Product Science Overview
Structure and Domains
CBX1 contains two main domains:
- N-terminal Chromodomain: This domain binds to histone proteins via methylated lysine residues, specifically recognizing and binding to histone H3 tails methylated at lysine 9 (H3K9me). This interaction is essential for the protein’s role in gene silencing and heterochromatin formation .
- C-terminal Chromo Shadow Domain (CSD): This domain is responsible for homodimerization and interaction with other chromatin-associated non-histone proteins. The CSD allows CBX1 to form complexes with other proteins, contributing to its function in chromatin organization .
Function
CBX1 is involved in several critical cellular processes:
- Epigenetic Repression: By binding to methylated histone H3 tails, CBX1 contributes to the formation of heterochromatin, leading to the repression of gene expression. This process is vital for maintaining the stability of the genome and regulating gene activity .
- Chromatin Organization: CBX1 interacts with the lamin B receptor (LBR), which helps anchor heterochromatin to the inner nuclear membrane. This interaction is crucial for the spatial organization of chromatin within the nucleus .
- Gene Silencing: CBX1 plays a role in gene silencing by recognizing and binding to specific methylated histone marks, thereby recruiting other proteins involved in chromatin remodeling and gene repression .
Clinical Significance
Mutations or dysregulation of CBX1 have been associated with various diseases, including:
Research and Applications
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