CASQ2 Human

Calsequestrin-2 Human Recombinant
Cat. No.
BT29514
Source
Escherichia Coli.
Synonyms
PDIB2, CASQ2, Calsequestrin-2, Calsequestrin cardiac muscle isoform, FLJ26321, FLJ93514.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CASQ2 Human Recombinant fused to 37 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 417 amino acids (20-399 a.a.) and having a molecular mass of 48.4 kDa. The CASQ2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
CASQ2, a member of the calsequestrin family, resides in the sarcoplasmic reticulum of cardiac and slow skeletal muscle cells. As a calcium-binding protein, it stores calcium essential for muscle function. The release of CASQ2-bound calcium through a calcium release channel triggers muscle contraction. CASQ2 possesses the capacity to bind 40 to 50 moles of calcium. Mutations in CASQ2 are implicated in stress-induced polymorphic ventricular tachycardia, also known as catecholaminergic polymorphic ventricular tachycardia 2, characterized by bidirectional ventricular tachycardia leading to cardiac arrest.
Description
Recombinant human CASQ2, with a 37 amino acid His Tag fused at its N-terminal, is produced in E. coli. This single, non-glycosylated polypeptide chain comprises 417 amino acids (20-399 a.a.) and exhibits a molecular mass of 48.4 kDa. The purification of CASQ2 is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has undergone sterile filtration.
Formulation
The CASQ2 solution is formulated with 20mM Tris-HCl (pH 8), 0.1M NaCl, 1mM DTT, and 20% glycerol.
Stability
For optimal storage, refrigerate at 4°C if the entire vial will be consumed within 2-4 weeks. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95% as determined by SDS-PAGE analysis.
Synonyms
PDIB2, CASQ2, Calsequestrin-2, Calsequestrin cardiac muscle isoform, FLJ26321, FLJ93514.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMEEG LNFPTYDGKD RVVSLSEKNF KQVLKKYDLL CLYYHEPVSS DKVTQKQFQL KEIVLELVAQ VLEHKAIGFV MVDAKKEAKL AKKLGFDEEG SLYILKGDRT IEFDGEFAAD VLVEFLLDLI EDPVEIISSK LEVQAFERIE DYIKLIGFFK SEDSEYYKAF EEAAEHFQPY IKFFATFDKG VAKKLSLKMN EVDFYEPFMD EPIAIPNKPY TEEELVEFVK EHQRPTLRRL RPEEMFETWE DDLNGIHIVA FAEKSDPDGY EFLEILKQVA RDNTDNPDLS ILWIDPDDFP LLVAYWEKTF KIDLFRPQIG VVNVTDADSV WMEIPDDDDL PTAEELEDWI EDVLSGKINT EDDDEDDDDD DNSDEEDNDD SDDDDDE.

Product Science Overview

Introduction

Calsequestrin-2 (CASQ2) is a calcium-binding protein predominantly found in cardiac muscle and slow-twitch skeletal muscle. It plays a crucial role in calcium regulation within the sarcoplasmic reticulum (SR), a specialized type of endoplasmic reticulum in muscle cells. CASQ2 is essential for proper muscle function, particularly in the heart, where it helps maintain calcium homeostasis and supports the excitation-contraction coupling process .

Structure and Function

CASQ2 is a high-capacity, low-affinity calcium-binding protein. Each molecule of CASQ2 can bind between 18 to 50 calcium ions, which it holds within the SR. This ability to store large amounts of calcium is vital for muscle contraction and relaxation cycles. The protein’s structure allows it to polymerize in response to changing calcium levels, enhancing its calcium-buffering capacity .

Role in Cardiac Function

In the heart, CASQ2 is integral to the regulation of calcium release from the SR. It interacts with the ryanodine receptor (RyR2), the primary calcium release channel in cardiac muscle cells. By modulating RyR2 activity, CASQ2 helps ensure the precise timing and amount of calcium released during each heartbeat. This regulation is crucial for maintaining a regular heart rhythm and preventing arrhythmias .

Genetic Mutations and Associated Disorders

Mutations in the CASQ2 gene have been linked to various cardiac disorders, including catecholaminergic polymorphic ventricular tachycardia (CPVT). CPVT is a potentially life-threatening condition characterized by abnormal heart rhythms triggered by physical activity or emotional stress. These mutations can disrupt the normal function of CASQ2, leading to impaired calcium handling and increased susceptibility to arrhythmias .

Human Recombinant CASQ2

Human recombinant CASQ2 is a laboratory-produced version of the protein, created using recombinant DNA technology. This technology allows for the production of large quantities of CASQ2, which can be used for research and therapeutic purposes. Recombinant CASQ2 is valuable for studying the protein’s function, understanding the mechanisms underlying related cardiac disorders, and developing potential treatments .

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