CASQ2 Antibody
Calsequestrin-2, Mouse Anti Human
Cat. No.
BT13909
Source
Synonyms
PDIB2, CASQ2, Calsequestrin-2, Calsequestrin cardiac muscle isoform, FLJ26321, FLJ93514.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
CASQ2, a member of the calsequestrin family, resides in the sarcoplasmic reticulum of cardiac and slow skeletal muscle cells. As a calcium-binding protein, it stores calcium essential for muscle function. Upon release through calcium channels, the calcium bound to CASQ2 triggers muscle contraction. Notably, CASQ2 can bind 40 to 50 moles of calcium. Mutations in CASQ2 are linked to stress-induced polymorphic ventricular tachycardia, also known as catecholaminergic polymorphic ventricular tachycardia 2, characterized by bidirectional ventricular tachycardia leading to cardiac arrest.
Formulation
The antibody is supplied as a 1 mg/ml solution in a buffer consisting of PBS at pH 7.4, 0.02% sodium azide (preservative), and 10% glycerol (stabilizer).
Storage Procedures
For short-term storage (up to 1 month), store the antibody at 4°C. For extended storage, it is recommended to store at -20°C. To maintain stability, avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The CASQ2 antibody has undergone rigorous testing using ELISA and Western blot analysis to confirm its specificity and reactivity. However, optimal working dilutions may vary depending on the specific application. For Western blot analysis, a dilution range of 1:1000 is recommended. As a starting point for other applications, a dilution of 1:1000 is suggested.
Synonyms
PDIB2, CASQ2, Calsequestrin-2, Calsequestrin cardiac muscle isoform, FLJ26321, FLJ93514.
Purification Method
CASQ2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT4E10AT.
Immunogen
Anti-human CASQ2 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CASQ2 amino acids 20-399 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Introduction
Calsequestrin-2 (CASQ2) is a high-capacity, moderate-affinity calcium-binding protein predominantly found in the sarcoplasmic reticulum of cardiac and skeletal muscle cells. It plays a crucial role in calcium storage and release, which is essential for muscle contraction. The protein binds 40 to 50 moles of calcium ions, acting as an internal calcium reservoir. Defects in CASQ2 are associated with catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2), a stress-induced arrhythmogenic disorder that can lead to sudden cardiac death .
Preparation Methods
The preparation of mouse anti-human Calsequestrin-2 antibodies involves several steps:
- Immunogen Preparation: A recombinant fragment corresponding to human Calsequestrin-2 is produced. This fragment typically includes amino acids 124-398 of the protein .
- Immunization: Mice are immunized with the recombinant fragment to elicit an immune response. The immunization process involves multiple injections over a period of time to ensure a robust antibody response.
- Hybridoma Production: Spleen cells from the immunized mice are fused with myeloma cells to create hybridoma cells. These hybridoma cells are capable of producing monoclonal antibodies against Calsequestrin-2.
- Screening and Selection: Hybridoma cells are screened for the production of specific antibodies using techniques such as ELISA (Enzyme-Linked Immunosorbent Assay) and Western blotting. Positive clones are selected and expanded.
- Purification: The antibodies are purified from the culture supernatant using affinity chromatography. This process ensures high purity and specificity of the antibodies.
Analysis of Chemical Reactions
Calsequestrin-2 interacts with various proteins and ions within the muscle cells, influencing several biochemical pathways:
- Calcium Binding and Release: Calsequestrin-2 binds calcium ions within the sarcoplasmic reticulum. Upon muscle stimulation, calcium is released from Calsequestrin-2 through the ryanodine receptor, triggering muscle contraction .
- Interaction with Triadin and Junctin: Calsequestrin-2 forms a complex with triadin and junctin, which helps anchor it to the sarcoplasmic reticulum membrane. This complex is essential for the proper functioning of the calcium release channel .
- Role in Cardiac Function: Mutations in the CASQ2 gene can lead to CPVT2, characterized by stress-induced ventricular tachycardia. This condition highlights the importance of Calsequestrin-2 in maintaining cardiac rhythm and function .
Applications
Mouse anti-human Calsequestrin-2 antibodies are widely used in research and diagnostic applications:
- Western Blotting: These antibodies are used to detect Calsequestrin-2 in various tissue samples, providing insights into its expression levels and potential abnormalities .
- Immunohistochemistry: The antibodies are employed to visualize the localization of Calsequestrin-2 in tissue sections, aiding in the study of its distribution and role in different tissues .
- ELISA: Enzyme-linked immunosorbent assays using these antibodies help quantify Calsequestrin-2 levels in biological samples, facilitating research on its function and regulation .
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