CAPNS1 Human
Calpain, Small Subunit 1 Human Recombinant
Cat. No.
BT3167
Source
Escherichia Coli.
Synonyms
Calpain Small Subunit 1, CAPN4, Calcium-Activated Neutral Proteinase Small Subunit, Calcium-Dependent Protease Small Subunit 1, Calpain Regulatory Subunit, CANP Small Subunit, CDPS, CSS1, Calcium-Dependent Protease Small Subunit, Calcium-Dependent Protease Small Subunit, Calpain 4 Small Subunit (30K), Calpain Small Polypeptide, Calpain Small Subunit 1, CALPAIN4, CANPS, CAPNS, CANP, CAPNS1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CAPNS1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 208 amino acids (84-268) and having a molecular mass of 23.8 kDa.
CAPNS1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CAPNS1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Calpain, Small Subunit 1 (CAPNS1), a member of the calpain small subunit family, plays a crucial role in cellular regulation. Calpains, a family of calcium-dependent, cysteine proteases found ubiquitously in mammalian cells, are known to be involved in neurodegenerative processes due to their activation by calcium influx and oxidative stress. These proteases function as heterodimers, consisting of a large catalytic subunit (calpain 1 in Calpain I and calpain 2 in Calpain II) and a common small regulatory subunit encoded by the CAPNS1 gene. This small subunit, CAPNS1, is essential for the stability and activity of both calpain heterodimers, which are involved in various cellular processes such as apoptosis, proliferation, migration, adhesion, and autophagy.
Description
Recombinant human CAPNS1, produced in E. coli, is a non-glycosylated polypeptide chain comprising 208 amino acids (84-268) with a molecular weight of 23.8 kDa. The protein consists of a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The CAPNS1 solution is provided at a concentration of 0.5 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), store the CAPNS1 solution at 4°C. For extended storage, freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. To maintain protein integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of CAPNS1 is determined to be greater than 95.0% using SDS-PAGE analysis.
Synonyms
Calpain Small Subunit 1, CAPN4, Calcium-Activated Neutral Proteinase Small Subunit, Calcium-Dependent Protease Small Subunit 1, Calpain Regulatory Subunit, CANP Small Subunit, CDPS, CSS1, Calcium-Dependent Protease Small Subunit, Calcium-Dependent Protease Small Subunit, Calpain 4 Small Subunit (30K), Calpain Small Polypeptide, Calpain Small Subunit 1, CALPAIN4, CANPS, CAPNS, CANP, CAPNS1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSRTHYSNI EANESEEVRQ FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG TGQIQVNIQE WLQLTMYS.
Product Science Overview
Structure and Function
Calpain I and II are heterodimeric enzymes composed of distinct large subunits and a common small subunit . The small regulatory subunit, CSS1, consists of an N-terminal domain rich in glycine residues and a C-terminal calcium-binding domain . This structure is essential for the enzyme’s stability and function.
The activation of calpains is triggered by calcium influx and oxidative stress, which can lead to their involvement in neurodegenerative processes . Calpains have been implicated in various diseases, including pulmonary hypertension and spinal muscular atrophy .
Recombinant Human Calpain, Small Subunit 1
Recombinant Human Calpain, Small Subunit 1 is a fragment protein expressed in Escherichia coli . It is typically produced with high purity (>95%) and is suitable for SDS-PAGE analysis . This recombinant protein is used in research to study the function and regulation of calpains, as well as their role in disease mechanisms.
Applications in Research
The recombinant form of CSS1 is valuable in various research applications, including:
- Studying Calpain Activation: Understanding how calpains are activated and regulated in different cellular contexts.
- Disease Mechanisms: Investigating the role of calpains in diseases such as neurodegenerative disorders and muscular dystrophies.
- Drug Development: Screening for potential inhibitors of calpain activity that could be used as therapeutic agents.
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