CAPNS1 Antibody
Description
Product Specs
Product Science Overview
Calpain, Small Subunit 1 (CAPNS1), also known as Calpain regulatory subunit, is a crucial component of the calpain protease system. Calpains are calcium-dependent, non-lysosomal cysteine proteases involved in various cellular processes, including cell motility, cell cycle progression, and apoptosis. The small subunit, CAPNS1, is essential for the stability and activity of the calpain complex.
CAPNS1 is a regulatory subunit that forms a heterodimer with the large catalytic subunit of calpain. This heterodimerization is necessary for the protease activity of calpain. The small subunit contains EF-hand motifs, which are responsible for calcium binding, a critical factor for calpain activation. The interaction between the small and large subunits stabilizes the protease and allows it to respond to calcium signals within the cell.
Calpains, including CAPNS1, play a significant role in various physiological processes:
- Cytoskeletal Remodeling: Calpains are involved in the reorganization of the actin cytoskeleton, which is crucial for cell movement and shape changes.
- Signal Transduction: They participate in signal transduction pathways by modulating the activity of various signaling proteins.
- Apoptosis: Calpains can cleave and activate or inactivate key proteins involved in apoptosis, thus playing a role in programmed cell death.
- Cell Cycle Regulation: They are involved in the regulation of the cell cycle by processing cyclins and other cell cycle-related proteins.
Dysregulation of calpain activity, including that of CAPNS1, has been implicated in several diseases:
- Neurodegenerative Diseases: Overactivation of calpains can lead to the degradation of essential neuronal proteins, contributing to diseases like Alzheimer’s and Parkinson’s.
- Cancer: Calpains are involved in cancer progression by promoting cell migration, invasion, and metastasis.
- Muscular Dystrophies: Mutations in calpain genes can lead to muscular dystrophies, characterized by muscle weakness and degeneration.
The mouse anti-human CAPNS1 antibody is a monoclonal antibody designed to specifically bind to the human CAPNS1 protein. This antibody is widely used in research to study the expression and function of CAPNS1 in various biological contexts. It is suitable for applications such as Western blotting, immunohistochemistry, and immunoprecipitation.
- Western Blotting: The antibody can detect CAPNS1 in cell lysates, allowing researchers to study its expression levels under different conditions.
- Immunohistochemistry: It can be used to visualize the localization of CAPNS1 in tissue sections, providing insights into its role in various tissues.
- Immunoprecipitation: The antibody can be used to pull down CAPNS1 from cell extracts, enabling the study of its interacting partners and post-translational modifications.
